pdBPV-MMTneo(342-12)
pdBPV-MMTneo(342-12)
规格:
货期:
编号:TS175919
品牌:Testobio
| 产品名称： | pdBPV-MMTneo(342-12) |
|---|---|
| 商品货号： | TS175919 |
| Designations： | pdBPV-MMTneo(342-12) |
| Depositors： | PM Howley |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 14.6000003814697300 Vector: pdBPV-MMTneo(342-12) (plasmid) Promoters: Promoter MMT (mouse metallothionein I) Construction: pML2, BPV-1, Tn5, mouse Marker(s):G418R,ampR Construct size (kb): 14.60000038146973 Features: marker(s): G418R marker(s): ampR other: SV40 splicing and 3 processing signals promoter: MMT (mouse metallothionein I) replicon: complete BPV-1 genome replicon: pMB1 restriction site: BamHI restriction site: BglII restriction site: EcoRI |
| Applications： | shuttle vector vector permitting positive selection for integration |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--7.8, 4.0, 2.4, 0.7; EcoRI--9.4, 4.8; HindIII--8.6, 6.4. A stable extrachromosomal plasmid which can be positively selected for in mouse cells. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Law MF, et al. A stable bovine papillomavirus hybrid plasmid that expresses a dominant selective trait. Mol. Cell. Biol. 3: 2110-2115, 1983. PubMed: 6318082 |
| Shipped： | freeze-dried |
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| 产品名称： | pdBPV-MMTneo(342-12) |
|---|---|
| 商品货号： | TS175919 |
| Designations： | pdBPV-MMTneo(342-12) |
| Depositors： | PM Howley |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 14.6000003814697300 Vector: pdBPV-MMTneo(342-12) (plasmid) Promoters: Promoter MMT (mouse metallothionein I) Construction: pML2, BPV-1, Tn5, mouse Marker(s):G418R,ampR Construct size (kb): 14.60000038146973 Features: marker(s): G418R marker(s): ampR other: SV40 splicing and 3 processing signals promoter: MMT (mouse metallothionein I) replicon: complete BPV-1 genome replicon: pMB1 restriction site: BamHI restriction site: BglII restriction site: EcoRI |
| Applications： | shuttle vector vector permitting positive selection for integration |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--7.8, 4.0, 2.4, 0.7; EcoRI--9.4, 4.8; HindIII--8.6, 6.4. A stable extrachromosomal plasmid which can be positively selected for in mouse cells. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Law MF, et al. A stable bovine papillomavirus hybrid plasmid that expresses a dominant selective trait. Mol. Cell. Biol. 3: 2110-2115, 1983. PubMed: 6318082 |
| Shipped： | freeze-dried |
