| 产品名称： | pGEM4Z-AMT7 |
|---|---|
| 商品货号： | TS176036 |
| Designations： | pGEM4Z-AMT7 |
| Species： | Homo sapiens, human |
| Depositors： | GI Bell |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: skeletal muscle Gene product: solute carrier family 2 (facilitated glucose transporter), member 4(glucose transporter 4) GLUT4 Alleles: A1, A2, A1, A2 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--2.9, 2.1; EcoRI--2.9, 2.1; HindIII--5.0; BamHI--5.0, PstI--3.3, 0.7, 0.5, 0.5. The insert includes these restriction sites (nucleotide position according to the published sequence): PstI--251, 788, 1025, 1739, 1791; BglI--516, 882, 1212; SacI--1370; XhoI--851, 1557. There is an unreported EcoRI site at the 3 end of the insert. GLUT4 is the insulin-regulated adipose/muscle type of transporter. This construct was linearized with HindIII and transcribed using SP6 RNA polymerase. The product of the in vitro translation of the transcript was recognized by monoclonal antibody 1F8 which recognizes the insulin-regulatable glucose transporter. The insert contains the complete coding sequence, including nucleotides 122-2128 of the published sequence. The KpnI polymorphism was reported using phJHT-3 which includes nucleotides 1-1472 of the published sequence. Enzyme(s) not detecting polymorphism: PvuII, SacI, StuI, HindIII, XhoI, AvaI, AccI, AvaII, Eco0109; BglII, BglI, HincII, MspI, StyI, HinfI, MboII, RsaI, Sau3A, SacI, SspI, TaqI. |
| References： | Fukumoto H, et al. Cloning and characterization of the major insulin-responsive glucose transporter expressed in human skeletal muscle and other insulin-responsive tissues. J. Biol. Chem. 264: 7776-7779, 1989. PubMed: 2656669 Leysens N, et al. A KpnI polymorphism for the human insulin-responsive glucose transporter gene (GLUT4) on chromosome 17. Nucleic Acids Res. 17: 3621, 1989. PubMed: 2566978 Bell GI, et al. Polymorphic human insulin-responsive glucose-transporter gene on chromosome 17p13. Diabetes 38: 1072-1075, 1989. PubMed: 2568955 Graeme I Bell, personal communication |
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| 产品名称： | pGEM4Z-AMT7 |
|---|---|
| 商品货号： | TS176036 |
| Designations： | pGEM4Z-AMT7 |
| Species： | Homo sapiens, human |
| Depositors： | GI Bell |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: skeletal muscle Gene product: solute carrier family 2 (facilitated glucose transporter), member 4(glucose transporter 4) GLUT4 Alleles: A1, A2, A1, A2 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--2.9, 2.1; EcoRI--2.9, 2.1; HindIII--5.0; BamHI--5.0, PstI--3.3, 0.7, 0.5, 0.5. The insert includes these restriction sites (nucleotide position according to the published sequence): PstI--251, 788, 1025, 1739, 1791; BglI--516, 882, 1212; SacI--1370; XhoI--851, 1557. There is an unreported EcoRI site at the 3 end of the insert. GLUT4 is the insulin-regulated adipose/muscle type of transporter. This construct was linearized with HindIII and transcribed using SP6 RNA polymerase. The product of the in vitro translation of the transcript was recognized by monoclonal antibody 1F8 which recognizes the insulin-regulatable glucose transporter. The insert contains the complete coding sequence, including nucleotides 122-2128 of the published sequence. The KpnI polymorphism was reported using phJHT-3 which includes nucleotides 1-1472 of the published sequence. Enzyme(s) not detecting polymorphism: PvuII, SacI, StuI, HindIII, XhoI, AvaI, AccI, AvaII, Eco0109; BglII, BglI, HincII, MspI, StyI, HinfI, MboII, RsaI, Sau3A, SacI, SspI, TaqI. |
| References： | Fukumoto H, et al. Cloning and characterization of the major insulin-responsive glucose transporter expressed in human skeletal muscle and other insulin-responsive tissues. J. Biol. Chem. 264: 7776-7779, 1989. PubMed: 2656669 Leysens N, et al. A KpnI polymorphism for the human insulin-responsive glucose transporter gene (GLUT4) on chromosome 17. Nucleic Acids Res. 17: 3621, 1989. PubMed: 2566978 Bell GI, et al. Polymorphic human insulin-responsive glucose-transporter gene on chromosome 17p13. Diabetes 38: 1072-1075, 1989. PubMed: 2568955 Graeme I Bell, personal communication |
