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hPAR2 cDNA
hPAR2 cDNA
规格:
货期:
编号:TS176430
品牌:Testobio
产品名称: hPAR2 cDNA
商品货号: TS176430
Designations: hPAR2 cDNA
GenBank Number:

Z49993

Z49994

Species: Homo sapiens, human
Depositors: JB Sundelin
Applications:
in another host, produces protein G-protein coupled receptor 11 proteinase-activated receptor 2
Vector:
Construct size (kb): 5.360000133514404
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSG5
Intact vector size: 4.076
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI BamHI
Polylinker sites: EcoRI BamHI BglII
Construction: pKCR2, Bluescribe M13+
Host range: eukaryotic cells; Escherichia coli
Features (with orientation and position when available):
restriction site: SalI
promoter for expression: SV40 early, ->
other: rabbit beta-globin intron II, ->
promoter for in vitro transcription: T7, ->
MCS: EcoRI...BglII, ->
terminator: SV40 polyadenylation, ->
replicon: pMB1, marker(s): ampR, replicon: M13, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GPR11
Other gene symbols: PAR2*
Gene name: G-protein coupled receptor 11
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q13
Tissue: stomach
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 1.283
Cross references: DNA Seq. Acc.: Z49993
Nucleotides 1-91 of the insert correspond to
nucleotides 195-285 of Z49993.
DNA Seq. Acc.: Z49994
Nucleotides 92-1283 of the insert correspond to
nucleotides 97-1288 of Z49994.
Insert lengths(kb): 1.282999992370606
Tissue: stomach
Gene product: G-protein coupled receptor 11(proteinase-activated receptor 2) GPR11
Target Gene: G-protein coupled receptor 11
Insert Size (kb): 1.283
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--4.1, 1.3; EcoRI--5.4; BglI--2.1, 1.6, 1.1, 0.45.
Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.35; NcoI--0.55, 0.83; PstI--0.07; PvuII--1.02.
Insert was amplified by PCR of total RNA from human stomach, followed by restriction digestion with BamHI.
References:

Nystedt S, et al. Molecular cloning and functional expression of the gene encoding the human proteinase-activated receptor2. Eur. J. Biochem. 232: 84-89, 1995. PubMed: 7556175

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hPAR2 cDNA

  • 货号: TS176430
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: hPAR2 cDNA
商品货号: TS176430
Designations: hPAR2 cDNA
GenBank Number:

Z49993

Z49994

Species: Homo sapiens, human
Depositors: JB Sundelin
Applications:
in another host, produces protein G-protein coupled receptor 11 proteinase-activated receptor 2
Vector:
Construct size (kb): 5.360000133514404
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSG5
Intact vector size: 4.076
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI BamHI
Polylinker sites: EcoRI BamHI BglII
Construction: pKCR2, Bluescribe M13+
Host range: eukaryotic cells; Escherichia coli
Features (with orientation and position when available):
restriction site: SalI
promoter for expression: SV40 early, ->
other: rabbit beta-globin intron II, ->
promoter for in vitro transcription: T7, ->
MCS: EcoRI...BglII, ->
terminator: SV40 polyadenylation, ->
replicon: pMB1, marker(s): ampR, replicon: M13, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GPR11
Other gene symbols: PAR2*
Gene name: G-protein coupled receptor 11
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q13
Tissue: stomach
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 1.283
Cross references: DNA Seq. Acc.: Z49993
Nucleotides 1-91 of the insert correspond to
nucleotides 195-285 of Z49993.
DNA Seq. Acc.: Z49994
Nucleotides 92-1283 of the insert correspond to
nucleotides 97-1288 of Z49994.
Insert lengths(kb): 1.282999992370606
Tissue: stomach
Gene product: G-protein coupled receptor 11(proteinase-activated receptor 2) GPR11
Target Gene: G-protein coupled receptor 11
Insert Size (kb): 1.283
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--4.1, 1.3; EcoRI--5.4; BglI--2.1, 1.6, 1.1, 0.45.
Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.35; NcoI--0.55, 0.83; PstI--0.07; PvuII--1.02.
Insert was amplified by PCR of total RNA from human stomach, followed by restriction digestion with BamHI.
References:

Nystedt S, et al. Molecular cloning and functional expression of the gene encoding the human proteinase-activated receptor2. Eur. J. Biochem. 232: 84-89, 1995. PubMed: 7556175

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