The Ac 711 cell line was derived from the mammary tumor of a transgenic mouse carrying thexa0 Zeta-Globin-v-Ha-ras transgene.

The xa0Ac 711 cell line was derived from the mammary tumor of a transgenic mouse carrying thexa0 Zeta-Globin-v-Ha-ras transgene.xa0

Ras proteins are essential components of signaling networks controlling cellular proliferation, differentiation and survival.xa0

Over expression of Ha-Ras has been shown to be correlated with tumor progression, and cell transformation.xa0xa0

The cell line Ac 711 overexpresses Ha-Ras. It is useful in the studies of the activation and requirement for Ras signal transduction pathways in mammary gland tumors.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 8 X 10⁴ to 1.5 X 10⁵ viable cells/cm² is recommended.
6. Incubate cultures at 37°C. Subculture when the cell concentration is between 3 X 10⁵ to 4 X 10⁵ cells/cm².

Muller WJ, et al. Single-step induction of mammary adenocarcinoma in transgenic mice bearing the activated c-neu oncogene. Cell 54(1): 105-15, 1988 PubMed: 2898299

Elson A, et al. Protein-tyrosine phosphatase epsilon. An isoform specifically expressed in mouse mammary tumors initiated by v-Ha-ras OR neu. J. Biol. Chem. 270(44): 26116-26122, 1995 PubMed: 7592814

Amundadottir LT, et al. Signal transduction pathways activated and required for mammary carcinogenesis in response to specific oncogenes. Oncogene 16(6): 737-746, 1998 PubMed:9488037

Fantin VR, et al. A novel mitochondriotoxic small molecule that selectively inhibits tumor cell growth. Cancer Cell 2(1): 29-42, 2002 PubMed: 12150823