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hMR(1-516)
hMR(1-516)
规格:
货期:
编号:TS177128
品牌:Testobio
产品名称: hMR(1-516)
商品货号: TS177128
Designations: hMR(1-516)
GenBank Number:

M80582

Species: Homo sapiens, human
Vector:
Construct size (kb): 6.619999885559082
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: mLR
Genomic copy number: unique
Gene name: mineralocorticoid receptor (aldosterone receptor)
Contains complete coding sequence?: U
Chromosome: 4; Localization: 4 q31; 4, cR
Tissue: lymphocyte
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 3.677
Cross references: DNA Seq. Acc.: M80582
Nucleotides 1-3677 of the insert correspond to
nucleotides 1-3677 of M80582.
Insert lengths(kb): 3.677000045776367
Tissue: lymphocyte
Gene product: mineralocorticoid receptor (aldosterone receptor) MLR
Insert Size (kb): 3.677
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 3.4; EcoRI-- 4.0, 3.0; HindIII--4.3, 2.7; KpnI--4.4, 2.5; XbaI--4.5, 2.3.
The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--3.17; BglII--1.46; HindIII--1.0; KpnI--1.34; NcoI--0.67, 2.43; PstI--2.96; PvuII--2.54; SacI--2.63; XbaI--2.16.
Contains regulatory elements and an open reading frame for aa 1-516 (nt 2124-3677), terminating at the EcoRI site.
References:

Govindan MV, et al. Differential regulation of mouse mammary tumor virus-bacterial chloramphenicol acetyltransferase chimeric gene by human mineralocorticoid hormone-receptor complexes. J. Steroid Biochem. Mol. Biol. 39: 91-103, 1991. PubMed: 1648951

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hMR(1-516)

  • 货号: TS177128
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  • 品牌 : TESTOBIO
产品名称: hMR(1-516)
商品货号: TS177128
Designations: hMR(1-516)
GenBank Number:

M80582

Species: Homo sapiens, human
Vector:
Construct size (kb): 6.619999885559082
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: mLR
Genomic copy number: unique
Gene name: mineralocorticoid receptor (aldosterone receptor)
Contains complete coding sequence?: U
Chromosome: 4; Localization: 4 q31; 4, cR
Tissue: lymphocyte
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 3.677
Cross references: DNA Seq. Acc.: M80582
Nucleotides 1-3677 of the insert correspond to
nucleotides 1-3677 of M80582.
Insert lengths(kb): 3.677000045776367
Tissue: lymphocyte
Gene product: mineralocorticoid receptor (aldosterone receptor) MLR
Insert Size (kb): 3.677
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 3.4; EcoRI-- 4.0, 3.0; HindIII--4.3, 2.7; KpnI--4.4, 2.5; XbaI--4.5, 2.3.
The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--3.17; BglII--1.46; HindIII--1.0; KpnI--1.34; NcoI--0.67, 2.43; PstI--2.96; PvuII--2.54; SacI--2.63; XbaI--2.16.
Contains regulatory elements and an open reading frame for aa 1-516 (nt 2124-3677), terminating at the EcoRI site.
References:

Govindan MV, et al. Differential regulation of mouse mammary tumor virus-bacterial chloramphenicol acetyltransferase chimeric gene by human mineralocorticoid hormone-receptor complexes. J. Steroid Biochem. Mol. Biol. 39: 91-103, 1991. PubMed: 1648951

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