CCD-1141Sk

规格:

货期:

编号:TS177373

品牌:Testobio

产品名称：	CCD-1141Sk
商品货号：	TS177373
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	44 years
Gender：	male
Ethnicity：	Caucasian
Applications：	The line was established from normal skin taken from the abdomen.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The line was established from normal skin taken from the abdomen.
Clinical Data：	male Caucasian 44 years
Comments：	The line was established from normal skin taken from the abdomen. Cells senesce after approximately 50 population doublings.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Name of Depositor：	F.Haq, ATCC
Year of Origin：	August, 2003

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CCD-1141Sk

货号: TS177373
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产品名称：	CCD-1141Sk
商品货号：	TS177373
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	44 years
Gender：	male
Ethnicity：	Caucasian
Applications：	The line was established from normal skin taken from the abdomen.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The line was established from normal skin taken from the abdomen.
Clinical Data：	male Caucasian 44 years
Comments：	The line was established from normal skin taken from the abdomen. Cells senesce after approximately 50 population doublings.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Name of Depositor：	F.Haq, ATCC
Year of Origin：	August, 2003