| 产品名称： | pNEB671 175-1B1, NEB671, V174-1B1 |
|---|---|
| 商品货号： | TS177574 |
| Designations： | pNEB671 175-1B1, NEB671, V174-1B1 |
| GenBank Number： | M74198 |
| Species： | Thermococcus litoralis Neuner et al. |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | produces protein DNA polymerase (Vent) |
| Vector： | Construct size (kb): 10.10 |
| Insert： | DNA: genomic Insert lengths(kb): 3.90 Gene product: DNA polymerase (Vent) pol Target Gene: DNA polymerase (Vent) |
| Insert Size (kb)： | 3.900 |
| Media： | ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of pNEB671 give the following sizes (kb): BamHI--10.5; BamHI/NdeI--6.2, 2.1 doublet; EcoRI--9.0, 1.9; HindIII--9.0, 0.7, 0.7. pLysS is also detected at 4.9 kb (or 4.2 and 0.6 kb with BamHI). Constructed by modifying the sequence TTTATG to CATATG to create a NdeI site at the 5 end (nt 288-293), and by removing the intervening sequence spanning nucleotides 1776 and 3398 of the sequence record. |
| References： | Comb DG, et al. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,322,785 dated Jun 21 1994 Comb DG, et al. Recombinant thermostable DNA polymerase from archaebacteria. US Patent 5,352,778 dated Oct 4 1994 Perler FB, et al. Intervening sequences in an Archaea DNA polymerase gene. Proc. Natl. Acad. Sci. USA 89: 5577-5581, 1992. PubMed: 1608969 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pNEB671 175-1B1, NEB671, V174-1B1 |
|---|---|
| 商品货号： | TS177574 |
| Designations： | pNEB671 175-1B1, NEB671, V174-1B1 |
| GenBank Number： | M74198 |
| Species： | Thermococcus litoralis Neuner et al. |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | produces protein DNA polymerase (Vent) |
| Vector： | Construct size (kb): 10.10 |
| Insert： | DNA: genomic Insert lengths(kb): 3.90 Gene product: DNA polymerase (Vent) pol Target Gene: DNA polymerase (Vent) |
| Insert Size (kb)： | 3.900 |
| Media： | ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of pNEB671 give the following sizes (kb): BamHI--10.5; BamHI/NdeI--6.2, 2.1 doublet; EcoRI--9.0, 1.9; HindIII--9.0, 0.7, 0.7. pLysS is also detected at 4.9 kb (or 4.2 and 0.6 kb with BamHI). Constructed by modifying the sequence TTTATG to CATATG to create a NdeI site at the 5 end (nt 288-293), and by removing the intervening sequence spanning nucleotides 1776 and 3398 of the sequence record. |
| References： | Comb DG, et al. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,322,785 dated Jun 21 1994 Comb DG, et al. Recombinant thermostable DNA polymerase from archaebacteria. US Patent 5,352,778 dated Oct 4 1994 Perler FB, et al. Intervening sequences in an Archaea DNA polymerase gene. Proc. Natl. Acad. Sci. USA 89: 5577-5581, 1992. PubMed: 1608969 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
