| 产品名称： | LL08NS02 |
|---|---|
| 商品货号： | TS178099 |
| Designations： | LL08NS02 |
| Species： | Homo sapiens, human |
| Depositors： | M Van Dilla, JA Garnes |
| Origin： | hamster (CHO)/human lymphocyte UV20HL21-17 hybrid |
| Vector： | Tissue: hamster (CHO)/human lymphocyte UV20HL21-17 hybrid Chromosome: 8 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 20.0 Coverage: Clones for "complete" coverage: 24000.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen (volume: 0.2 ml) |
| Comments： | The peak was well-isolated from hamster background; little debris. According to fluorescence in situ hybridization using genomic hamster DNA with sorted chromosomes, there is 15% hamster DNA. About 84% of the library is human (and 16% hamster) by plaque hybridization. Of 52 isolates giving definitive results, 5 contained hamster sequences. One hybridized to hamster (2 fragments) and human (same size as insert) DNA. Of 76 inserts recloned into pUC13: 8-no identifiable insert; 4-very small inserts; 38-human repeats; 3-identical to others; 2--lost; 5-hamster; 7-no hybridization; 9-human (2 multiple copy, 4/7 single copy detect RFLP, 1 on chromosome 4. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 93. |
| References： | . Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986. Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319 Wood S, et al. Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP). Am. J. Hum. Genet. 39: 744-750, 1986. PubMed: 2879441 Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320 Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712 Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744 Marvin Van Dilla, personal communication |
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| 产品名称： | LL08NS02 |
|---|---|
| 商品货号： | TS178099 |
| Designations： | LL08NS02 |
| Species： | Homo sapiens, human |
| Depositors： | M Van Dilla, JA Garnes |
| Origin： | hamster (CHO)/human lymphocyte UV20HL21-17 hybrid |
| Vector： | Tissue: hamster (CHO)/human lymphocyte UV20HL21-17 hybrid Chromosome: 8 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 20.0 Coverage: Clones for "complete" coverage: 24000.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen (volume: 0.2 ml) |
| Comments： | The peak was well-isolated from hamster background; little debris. According to fluorescence in situ hybridization using genomic hamster DNA with sorted chromosomes, there is 15% hamster DNA. About 84% of the library is human (and 16% hamster) by plaque hybridization. Of 52 isolates giving definitive results, 5 contained hamster sequences. One hybridized to hamster (2 fragments) and human (same size as insert) DNA. Of 76 inserts recloned into pUC13: 8-no identifiable insert; 4-very small inserts; 38-human repeats; 3-identical to others; 2--lost; 5-hamster; 7-no hybridization; 9-human (2 multiple copy, 4/7 single copy detect RFLP, 1 on chromosome 4. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 93. |
| References： | . Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986. Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319 Wood S, et al. Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP). Am. J. Hum. Genet. 39: 744-750, 1986. PubMed: 2879441 Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320 Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712 Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744 Marvin Van Dilla, personal communication |
