pJWK20
pJWK20
规格:
货期:
编号:TS178486
品牌:Testobio
| 产品名称： | pJWK20 |
|---|---|
| 商品货号： | TS178486 |
| Designations： | pJWK20 |
| Species： | Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille |
| Depositors： | The University of Washington, JF Ryan, The University of Washington |
| Applications： | produces protein crystal protein (delta endotoxin) |
| Vector： | Construct size (kb): 20.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus thuringiensis subsp. kurstaki Gene name: crystal protein (delta endotoxin) Contains complete coding sequence?: U Strain: HD-73 Type of DNA: genomic Insert end: BglII Insert end: BglII Insert size (kb): 16.2 Cross references: DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus thuringiensis subsp. kurstaki Gene name: crystal protein (delta endotoxin) Contains complete coding sequence?: U Strain: HD-73 Type of DNA: genomic Insert end: BglII Insert end: BglII Insert size (kb): 16.2 Cross references: Insert lengths(kb): 16.20000076293945 Gene product: crystal protein (delta endotoxin) Target Gene: crystal protein (delta endotoxin) |
| Insert Size (kb)： | 16.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Expressed protein recognized by antibody to crystal protein of strain HD-1. Insert derived from the 45 megadalton plasmid of the source strain. |
| References： | Schnepf HE, Whiteley HR. Cloning and expression of the Bacillus thuringiensis crystal protein. Proc. Natl. Acad. Sci. USA 78: 2893-2897, 1981. PubMed: 7019914 Schmepf HE, Whiteley HR. Bacillus thuringiensis crystal protein in Escherichia coli. US Patent 4,467,036 dated Aug 21 1984 Schanegl HE, Whiteley HR. Bacillus thuringiensis crystal protein in Escherichia coli. US Patent 4,448,885 dated May 15 1984 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pJWK20 |
|---|---|
| 商品货号： | TS178486 |
| Designations： | pJWK20 |
| Species： | Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille |
| Depositors： | The University of Washington, JF Ryan, The University of Washington |
| Applications： | produces protein crystal protein (delta endotoxin) |
| Vector： | Construct size (kb): 20.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus thuringiensis subsp. kurstaki Gene name: crystal protein (delta endotoxin) Contains complete coding sequence?: U Strain: HD-73 Type of DNA: genomic Insert end: BglII Insert end: BglII Insert size (kb): 16.2 Cross references: DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus thuringiensis subsp. kurstaki Gene name: crystal protein (delta endotoxin) Contains complete coding sequence?: U Strain: HD-73 Type of DNA: genomic Insert end: BglII Insert end: BglII Insert size (kb): 16.2 Cross references: Insert lengths(kb): 16.20000076293945 Gene product: crystal protein (delta endotoxin) Target Gene: crystal protein (delta endotoxin) |
| Insert Size (kb)： | 16.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Expressed protein recognized by antibody to crystal protein of strain HD-1. Insert derived from the 45 megadalton plasmid of the source strain. |
| References： | Schnepf HE, Whiteley HR. Cloning and expression of the Bacillus thuringiensis crystal protein. Proc. Natl. Acad. Sci. USA 78: 2893-2897, 1981. PubMed: 7019914 Schmepf HE, Whiteley HR. Bacillus thuringiensis crystal protein in Escherichia coli. US Patent 4,467,036 dated Aug 21 1984 Schanegl HE, Whiteley HR. Bacillus thuringiensis crystal protein in Escherichia coli. US Patent 4,448,885 dated May 15 1984 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
