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L10BIOBR-MAPKK
L10BIOBR-MAPKK
规格:
货期:
编号:TS178489
品牌:Testobio
产品名称： L10BIOBR-MAPKK
商品货号： TS178489
Organism： Mus musculus, mouse
Cell Type： melanocyte
Product Format： frozen
Morphology： melanocyte
Culture Properties： adherent
Biosafety Level： 2 Cells containing SV40 viral DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age： newborn
Strain： B10.BR
Applications：
tumor model
Storage Conditions： liquid nitrogen vapor phase
Images：
Derivation：
The L10BIOBR-MAPKK cell line (TS178489) was derived by infecting the immortalized murine melanocyte cell line, L10BIOBR, with pBABE which encodes a constitutively active MAPKK. The vector contains the SV40 viral DNA sequences and the puromycin resistance gene. The cells were selected in medium containing puromycin. The introduction of the MAPKK gene into melanocytes leads to tumorigenesis in nude mice, activation of the angiogenic switch and increased production of the proangiogenic factor, vascular endothelial growth factor (VEGF), and matrix metalloproteinases (MMPs). Activation of MAP kinase signaling may be an important pathway involved in melanoma transformation. Inhibition of MAP kinase signaling may be useful in the prevention and treatment of melanoma. The L10BIOBR-MAPKK cell line and the corresponding negative control, L10BIOBR-GFP (CRL-2770), are a model for melanoma tumorigenesis and signal transduction PubMed: 12514183.
Tumorigenic： Yes
Effects：
Yes, produces tumors in nude mice inoculated subcutaneously with 10(6) cells
RefGovindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183
Comments：
The L10BIOBR-MAPKK cell line (TS178489) was derived by infecting the immortalized murine melanocyte cell line, L10BIOBR, with pBABE which encodes a constitutively active MAPKK. The vector contains the SV40 viral DNA sequences and the puromycin resistance gene. The cells were selected in medium containing puromycin. The introduction of the MAPKK gene into melanocytes leads to tumorigenesis in nude mice, activation of the angiogenic switch and increased production of the proangiogenic factor, vascular endothelial growth factor (VEGF), and matrix metalloproteinases (MMPs). Activation of MAP kinase signaling may be an important pathway involved in melanoma transformation. Inhibition of MAP kinase signaling may be useful in the prevention and treatment of melanoma. The L10BIOBR-MAPKK cell line and the corresponding negative control, L10BIOBR-GFP (CRL-2770), are a model for melanoma tumorigenesis and signal transduction PubMed: 12514183.
Complete Growth Medium： Hams F10 medium supplemented with 50 ng/ml TPA (Sigma Catalogue No. P-8139) and 7% horse serum
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 5 X 10(3) to 7 X 10(3) viable cells/sq. cm. is recommended.
  6. Incubate cultures at 37°C.
Interval: Subculture when cells reach a concentration of 4 X 10(4) cells/sq. cm.
Subcultivation Ratio: A subcultivation of 1:6 to 1:8 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation：
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time： 24 hours
Name of Depositor： JL Arbiser
Year of Origin： January 1, 2002
References：

Govindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183

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L10BIOBR-MAPKK

  • 货号: TS178489
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： L10BIOBR-MAPKK
商品货号： TS178489
Organism： Mus musculus, mouse
Cell Type： melanocyte
Product Format： frozen
Morphology： melanocyte
Culture Properties： adherent
Biosafety Level： 2 Cells containing SV40 viral DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age： newborn
Strain： B10.BR
Applications：
tumor model
Storage Conditions： liquid nitrogen vapor phase
Images：
Derivation：
The L10BIOBR-MAPKK cell line (TS178489) was derived by infecting the immortalized murine melanocyte cell line, L10BIOBR, with pBABE which encodes a constitutively active MAPKK. The vector contains the SV40 viral DNA sequences and the puromycin resistance gene. The cells were selected in medium containing puromycin. The introduction of the MAPKK gene into melanocytes leads to tumorigenesis in nude mice, activation of the angiogenic switch and increased production of the proangiogenic factor, vascular endothelial growth factor (VEGF), and matrix metalloproteinases (MMPs). Activation of MAP kinase signaling may be an important pathway involved in melanoma transformation. Inhibition of MAP kinase signaling may be useful in the prevention and treatment of melanoma. The L10BIOBR-MAPKK cell line and the corresponding negative control, L10BIOBR-GFP (CRL-2770), are a model for melanoma tumorigenesis and signal transduction PubMed: 12514183.
Tumorigenic： Yes
Effects：
Yes, produces tumors in nude mice inoculated subcutaneously with 10(6) cells
RefGovindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183
Comments：
The L10BIOBR-MAPKK cell line (TS178489) was derived by infecting the immortalized murine melanocyte cell line, L10BIOBR, with pBABE which encodes a constitutively active MAPKK. The vector contains the SV40 viral DNA sequences and the puromycin resistance gene. The cells were selected in medium containing puromycin. The introduction of the MAPKK gene into melanocytes leads to tumorigenesis in nude mice, activation of the angiogenic switch and increased production of the proangiogenic factor, vascular endothelial growth factor (VEGF), and matrix metalloproteinases (MMPs). Activation of MAP kinase signaling may be an important pathway involved in melanoma transformation. Inhibition of MAP kinase signaling may be useful in the prevention and treatment of melanoma. The L10BIOBR-MAPKK cell line and the corresponding negative control, L10BIOBR-GFP (CRL-2770), are a model for melanoma tumorigenesis and signal transduction PubMed: 12514183.
Complete Growth Medium： Hams F10 medium supplemented with 50 ng/ml TPA (Sigma Catalogue No. P-8139) and 7% horse serum
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 5 X 10(3) to 7 X 10(3) viable cells/sq. cm. is recommended.
  6. Incubate cultures at 37°C.
Interval: Subculture when cells reach a concentration of 4 X 10(4) cells/sq. cm.
Subcultivation Ratio: A subcultivation of 1:6 to 1:8 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation：
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time： 24 hours
Name of Depositor： JL Arbiser
Year of Origin： January 1, 2002
References：

Govindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183

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