| 产品名称： | pCAV/NOT-IL-7R cDNA H1 |
|---|---|
| 商品货号： | TS179159 |
| Designations： | pCAV/NOT-IL-7R cDNA H1 |
| GenBank Number： | M29696 |
| Species： | Homo sapiens, human |
| Depositors： | Immunex Corporation, SG Hallquist, Immunex Corporation |
| Applications： | in another host, produces protein interleukin 7 receptor |
| Vector： | Vector size (kb): 5.7 Construct size (kb): 7.80 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.07 Tissue: fibroblast cell line WI-26VA4 Gene product: interleukin 7 receptor IL7R Target Gene: interleukin 7 receptor |
| Insert Size (kb)： | 2.070 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI--5.5, 2.3; BamHI--3.7, 2.3, 1.8; HindIII--5.0, 1.8, 0.9; PstI--4.3, 2.4, 0.6, 0.5; XbaI--7.2, 0.6. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.74; HindIII--1.49; NdeI--1.76; PstI--0.59, 1.58; XmnI--1.38, 1.5; XbaI--1.86. Detects a transcript of approximately 4.5 kb in human spleen, thymus and in the human cell line WI-26VA4. pDC302 is a mammalian expression vector containing promoter and enhancer sequences from SV40 and CMV, leader sequences from adenovirus 2 followed by a multiple cloning site, SV40 polyadenylation and termination signals and adenovirus genes VAI and VAII. |
| References： | Goodwin RG, et al. Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell 60: 941-951, 1990. PubMed: 2317865 U.S. Patent 5,543,320 dated Aug 6 1996 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pCAV/NOT-IL-7R cDNA H1 |
|---|---|
| 商品货号： | TS179159 |
| Designations： | pCAV/NOT-IL-7R cDNA H1 |
| GenBank Number： | M29696 |
| Species： | Homo sapiens, human |
| Depositors： | Immunex Corporation, SG Hallquist, Immunex Corporation |
| Applications： | in another host, produces protein interleukin 7 receptor |
| Vector： | Vector size (kb): 5.7 Construct size (kb): 7.80 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.07 Tissue: fibroblast cell line WI-26VA4 Gene product: interleukin 7 receptor IL7R Target Gene: interleukin 7 receptor |
| Insert Size (kb)： | 2.070 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI--5.5, 2.3; BamHI--3.7, 2.3, 1.8; HindIII--5.0, 1.8, 0.9; PstI--4.3, 2.4, 0.6, 0.5; XbaI--7.2, 0.6. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.74; HindIII--1.49; NdeI--1.76; PstI--0.59, 1.58; XmnI--1.38, 1.5; XbaI--1.86. Detects a transcript of approximately 4.5 kb in human spleen, thymus and in the human cell line WI-26VA4. pDC302 is a mammalian expression vector containing promoter and enhancer sequences from SV40 and CMV, leader sequences from adenovirus 2 followed by a multiple cloning site, SV40 polyadenylation and termination signals and adenovirus genes VAI and VAII. |
| References： | Goodwin RG, et al. Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell 60: 941-951, 1990. PubMed: 2317865 U.S. Patent 5,543,320 dated Aug 6 1996 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
