pHGH107
pHGH107
规格:
货期:
编号:TS179191
品牌:Testobio
| 产品名称： | pHGH107 |
|---|---|
| 商品货号： | TS179191 |
| Designations： | pHGH107 |
| Species： | Homo sapiens, human |
| Depositors： | Genentech, Inc. |
| Applications： | produces protein growth hormone 1 growth hormone GH1 |
| Vector： | Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGH6 Intact vector size: 4.600 Type of vector: plasmid Vector end: EcoRI Vector end: HindIII Cloning sites: EcoRI Polylinker sites: Construction: pBR322, pKB268 Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR promoter: lacUV5 replicon: pMB1 Cross references: DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGH6 Intact vector size: 4.600 Type of vector: plasmid Vector end: EcoRI Vector end: HindIII Cloning sites: EcoRI Polylinker sites: Construction: pBR322, pKB268 Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR promoter: lacUV5 replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: GH1 Genomic copy number: unique Gene name: growth hormone 1 Contains complete coding sequence?: U Chromosome: 17; Localization: 17 q22-q24 Type of DNA: cDNA Insert end: EcoRI Insert end: SmaI Insert size (kb): 0.591 Cross references: DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: GH1 Genomic copy number: unique Gene name: growth hormone 1 Contains complete coding sequence?: U Chromosome: 17; Localization: 17 q22-q24 Type of DNA: cDNA Insert end: EcoRI Insert end: SmaI Insert size (kb): 0.591 Cross references: Insert lengths(kb): 0.5910000205039978 Gene product: growth hormone 1(growth hormone GH1) GH1 Target Gene: growth hormone 1 |
| Insert Size (kb)： | 0.591 |
| Media： | ATCC® Medium 1083: LB medium for chi 1776 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.6. For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). The insert contains sequences encoding all 191 amino acids of human growth hormone. Contains an internal PstI, BglII and PvuII site. The vector (ATCC 31539, 40012) is pBR322 modified by the insertion of 2 lac UV5 promoters at the EcoRI site. These promoters regulate expression of the insert. |
| References： | Goeddel DV, Heyneker HL. Method of constructing a replicable cloning vehicle having quasi-synthetic genes. US Patent 4,342,832 dated Aug 3 1982 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pHGH107 |
|---|---|
| 商品货号： | TS179191 |
| Designations： | pHGH107 |
| Species： | Homo sapiens, human |
| Depositors： | Genentech, Inc. |
| Applications： | produces protein growth hormone 1 growth hormone GH1 |
| Vector： | Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGH6 Intact vector size: 4.600 Type of vector: plasmid Vector end: EcoRI Vector end: HindIII Cloning sites: EcoRI Polylinker sites: Construction: pBR322, pKB268 Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR promoter: lacUV5 replicon: pMB1 Cross references: DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGH6 Intact vector size: 4.600 Type of vector: plasmid Vector end: EcoRI Vector end: HindIII Cloning sites: EcoRI Polylinker sites: Construction: pBR322, pKB268 Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR promoter: lacUV5 replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: GH1 Genomic copy number: unique Gene name: growth hormone 1 Contains complete coding sequence?: U Chromosome: 17; Localization: 17 q22-q24 Type of DNA: cDNA Insert end: EcoRI Insert end: SmaI Insert size (kb): 0.591 Cross references: DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: GH1 Genomic copy number: unique Gene name: growth hormone 1 Contains complete coding sequence?: U Chromosome: 17; Localization: 17 q22-q24 Type of DNA: cDNA Insert end: EcoRI Insert end: SmaI Insert size (kb): 0.591 Cross references: Insert lengths(kb): 0.5910000205039978 Gene product: growth hormone 1(growth hormone GH1) GH1 Target Gene: growth hormone 1 |
| Insert Size (kb)： | 0.591 |
| Media： | ATCC® Medium 1083: LB medium for chi 1776 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.6. For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). The insert contains sequences encoding all 191 amino acids of human growth hormone. Contains an internal PstI, BglII and PvuII site. The vector (ATCC 31539, 40012) is pBR322 modified by the insertion of 2 lac UV5 promoters at the EcoRI site. These promoters regulate expression of the insert. |
| References： | Goeddel DV, Heyneker HL. Method of constructing a replicable cloning vehicle having quasi-synthetic genes. US Patent 4,342,832 dated Aug 3 1982 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
