| 产品名称： | pYNM67 |
|---|---|
| 商品货号： | TS179954 |
| Designations： | pYNM67 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | This probe has some lane background, but RFLPs observed under normal conditions of hybridization and washing. |
| Vector： | Construct size (kb): 6.5 |
| Insert： | DNA: genomic Insert lengths(kb): 1.600000023841858 Gene product: DNA Segment, single copy D17S29 Alleles: B1, A1, A1, B1, B2, B2, C1, C1, C2, C2, A2, A2 |
| Insert Size (kb)： | 1.600 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.5; PstI--4.7, 1.6; HindIII--6.5; BamHI--6.5; ClaI--6.5. Codominant segregation for the TaqI RFLP demonstrated in 46 3-generation families. Codominant segregation demonstrated in 40 3-generation families for the RsaI RFLP. RsaI also identifies two additional polymorphisms. TaqI detects a 2-allele RFLP. This probe has some lane background, but RFLPs observed under normal conditions of hybridization and washing. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, MspI, PstI, PvuII. |
| References： | Baker SJ, et al. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science 244: 217-221, 1989. PubMed: 2649981 Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050 Yusuke Nakamura, personal communication |
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| 产品名称： | pYNM67 |
|---|---|
| 商品货号： | TS179954 |
| Designations： | pYNM67 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | This probe has some lane background, but RFLPs observed under normal conditions of hybridization and washing. |
| Vector： | Construct size (kb): 6.5 |
| Insert： | DNA: genomic Insert lengths(kb): 1.600000023841858 Gene product: DNA Segment, single copy D17S29 Alleles: B1, A1, A1, B1, B2, B2, C1, C1, C2, C2, A2, A2 |
| Insert Size (kb)： | 1.600 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.5; PstI--4.7, 1.6; HindIII--6.5; BamHI--6.5; ClaI--6.5. Codominant segregation for the TaqI RFLP demonstrated in 46 3-generation families. Codominant segregation demonstrated in 40 3-generation families for the RsaI RFLP. RsaI also identifies two additional polymorphisms. TaqI detects a 2-allele RFLP. This probe has some lane background, but RFLPs observed under normal conditions of hybridization and washing. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, MspI, PstI, PvuII. |
| References： | Baker SJ, et al. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science 244: 217-221, 1989. PubMed: 2649981 Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050 Yusuke Nakamura, personal communication |
