| 产品名称： | pDF8 AG397 |
|---|---|
| 商品货号： | TS180380 |
| Designations： | pDF8 AG397 |
| GenBank Number： | M15686 |
| Species： | Staphylococcus simulans Kloos and Schleifer |
| Depositors： | Applied Microbiology, Inc. |
| Applications： | produces protein lysostaphin |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Gene product: lysostaphin Target Gene: lysostaphin |
| Insert Size (kb)： | 1.5 |
| Media： | 1179 plus chloramphenicol (5 mcg/ml)
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--7.0; NsiI--2.2, 1.9, 1.2, 0.94; SphI--5.6, 1.4; XbaI--7.0. The insert contains the following restriction sites (approximate kb from the 5 end): PvuII--0.43, 0.46, 0.50, 0.58, 0.62; SphI--1.23. TS180380 was deposited 3/17/95 to supplement ATCC 67077. Requests for ATCC 67077 should be referred to TS180380. Constructed by excising the insert of pRG5 (ATCC 67076) with an EcoRI/HindIII digest, and blunt-ending the vector and insert with the Klenow fragment of DNA polymerase before ligation. Contains the complete coding sequence (nt 245-1414 of the sequence record). |
| References： | Recsei PA. Expression of the cloned lysostaphin gene. US Patent 4,931,390 dated Jun 5 1990 Recsei PA, et al. Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans. Proc. Natl. Acad. Sci. USA 84: 1127-1131, 1987. PubMed: 3547405 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pDF8 AG397 |
|---|---|
| 商品货号： | TS180380 |
| Designations： | pDF8 AG397 |
| GenBank Number： | M15686 |
| Species： | Staphylococcus simulans Kloos and Schleifer |
| Depositors： | Applied Microbiology, Inc. |
| Applications： | produces protein lysostaphin |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Gene product: lysostaphin Target Gene: lysostaphin |
| Insert Size (kb)： | 1.5 |
| Media： | 1179 plus chloramphenicol (5 mcg/ml)
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--7.0; NsiI--2.2, 1.9, 1.2, 0.94; SphI--5.6, 1.4; XbaI--7.0. The insert contains the following restriction sites (approximate kb from the 5 end): PvuII--0.43, 0.46, 0.50, 0.58, 0.62; SphI--1.23. TS180380 was deposited 3/17/95 to supplement ATCC 67077. Requests for ATCC 67077 should be referred to TS180380. Constructed by excising the insert of pRG5 (ATCC 67076) with an EcoRI/HindIII digest, and blunt-ending the vector and insert with the Klenow fragment of DNA polymerase before ligation. Contains the complete coding sequence (nt 245-1414 of the sequence record). |
| References： | Recsei PA. Expression of the cloned lysostaphin gene. US Patent 4,931,390 dated Jun 5 1990 Recsei PA, et al. Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans. Proc. Natl. Acad. Sci. USA 84: 1127-1131, 1987. PubMed: 3547405 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
