| 产品名称： | Human rhinovirus 1A |
|---|---|
| 商品货号： | TS180903 |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus A |
| Deposited As： | Rhinovirus 1a |
| Agent： | Human rhinovirus 1A |
| Strain： | 2060 |
| Common Name： | Human rhinovirus 1A |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | VR-1559™ was created by solvent treating Mycoplasma positive VR-1364™, passaging it three times and finding no detectable Mycoplasma by PCR.xa0 VR-1364™ was derived by replenishment (passage) of NIAID reagent V-101-003-021 (VR-1110™) at the ATCC. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： |
H1 HeLa (ATCC® CRL-1958™)
|
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | ATCC, NIAID |
| Special Collection： | NCRR Contract |
| Source： | Naso-pharyngeal washings from patient with mild respiratory illness, Ohio. |
| References： | Mogabgab WJ, Pelon W. Problems in characterizing and identifying an apparently new virus found in association with mild respiratory disease in recuits. Ann NY Acad Sci 67(8): 403-412, 1957. PubMed: 13411976 Pelon W, et al. A cytopathic agent isolated from naval recruits with mild respiratory illnesses. Proc. Soc. Exp. Biol. Med. 94(2): 262-267, 1957. PubMed: 13408229 Pelon, W. Further studies on 2060 virus. Proc. Soc. Exp. Biol. Med. 102: 392-395, 1959. PubMed: 14431507 Pelon W. Classification of the "2060" virus as Echo 28 and further study of its properties. Am. J. Hyg. 73: 36-54, 1961. PubMed: 13734022 |
| Cross References： | Nucleotide (GenBank) : FJ445111.1 Human rhinovirus 1 strain TS180903, complete genome |
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| 产品名称： | Human rhinovirus 1A |
|---|---|
| 商品货号： | TS180903 |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus A |
| Deposited As： | Rhinovirus 1a |
| Agent： | Human rhinovirus 1A |
| Strain： | 2060 |
| Common Name： | Human rhinovirus 1A |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | VR-1559™ was created by solvent treating Mycoplasma positive VR-1364™, passaging it three times and finding no detectable Mycoplasma by PCR.xa0 VR-1364™ was derived by replenishment (passage) of NIAID reagent V-101-003-021 (VR-1110™) at the ATCC. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： |
H1 HeLa (ATCC® CRL-1958™)
|
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | ATCC, NIAID |
| Special Collection： | NCRR Contract |
| Source： | Naso-pharyngeal washings from patient with mild respiratory illness, Ohio. |
| References： | Mogabgab WJ, Pelon W. Problems in characterizing and identifying an apparently new virus found in association with mild respiratory disease in recuits. Ann NY Acad Sci 67(8): 403-412, 1957. PubMed: 13411976 Pelon W, et al. A cytopathic agent isolated from naval recruits with mild respiratory illnesses. Proc. Soc. Exp. Biol. Med. 94(2): 262-267, 1957. PubMed: 13408229 Pelon, W. Further studies on 2060 virus. Proc. Soc. Exp. Biol. Med. 102: 392-395, 1959. PubMed: 14431507 Pelon W. Classification of the "2060" virus as Echo 28 and further study of its properties. Am. J. Hyg. 73: 36-54, 1961. PubMed: 13734022 |
| Cross References： | Nucleotide (GenBank) : FJ445111.1 Human rhinovirus 1 strain TS180903, complete genome |
