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Bodo saliens Larsen and Patterson
Bodo saliens Larsen and Patterson
规格:
货期:
编号:TS181292
品牌:Testobio
产品名称： Bodo saliens Larsen and Patterson
商品货号： TS181292
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
seawater, Atlantic Ocean, Southampton, England, 1992
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Xenic
Type Strain： no
Medium： ATCC® Medium 1525: Seawater 802 medium
Growth Conditions：
Temperature: 10-25°C
Cryopreservation：

Cryoprotective Solution
DMSO, 2.0 ml
Fresh growth medium w/o bacteria, 8.0 ml

  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.
  3. Adjust the concentration of cells at least 2 x 106/ml in freshmedium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC® xa0700831).
  9. Incubate at 25°C with the cap screwed on tightly.
  10. Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 ml to 10.0 ml of bacterized ATCC medium 1525.
  11. Follow the protocol for maintenance of culture.
Name of Depositor： S Tong
Year of Origin： 1992
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Bodo saliens Larsen and Patterson

  • 货号: TS181292
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Bodo saliens Larsen and Patterson
商品货号： TS181292
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
seawater, Atlantic Ocean, Southampton, England, 1992
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Xenic
Type Strain： no
Medium： ATCC® Medium 1525: Seawater 802 medium
Growth Conditions：
Temperature: 10-25°C
Cryopreservation：

Cryoprotective Solution
DMSO, 2.0 ml
Fresh growth medium w/o bacteria, 8.0 ml

  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.
  3. Adjust the concentration of cells at least 2 x 106/ml in freshmedium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC® xa0700831).
  9. Incubate at 25°C with the cap screwed on tightly.
  10. Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 ml to 10.0 ml of bacterized ATCC medium 1525.
  11. Follow the protocol for maintenance of culture.
Name of Depositor： S Tong
Year of Origin： 1992
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周雪：13372109301
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地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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