| 产品名称： | Human rhinovirus 17 |
|---|---|
| 商品货号： | TS181430 |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus B |
| Agent： | Human rhinovirus 17 |
| Strain： | 33342 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Human rhinovirus 17, complete genome |
| Comments： | ATCC®xa0VR-1663™ was treated with diethyl ether at ATCC® to remove Mycoplasma. The previous item number was ATCC®xa0VR-1127™ (NIAID Research Reference Reagent V-106-003-021). Strain 33342 is the prototype strain for Human rhinovirus 17. |
| Effect on Host： | CPE, rounding, sloughing |
| Recommended Host： | H1HeLa (ATCC® CRL-1958™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and then wash monolayer with PBS or serum free medium prior to inoculation. Inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb for 1 hour at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 3 days at 33°C in a humidified 5% CO2 atmosphere while rocking, until CPE is progressed throughout |
| Name of Depositor： | NIAID, ATCC |
| Source： | Presumed from throat swab from adult with upper respiratory illness, North Carolina, 1959 |
| References： | Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967. Tapparel C, et al. New complete genome sequences of human rhinoviruses shed light on their phylogeny and genomic features. BMC Genomics 8: 224, 2007. PubMed: 17623054 |
| Cross References： | Nucleotide (GenBank) : EF173420.1 Human rhinovirus 17, complete genome |
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| 产品名称： | Human rhinovirus 17 |
|---|---|
| 商品货号： | TS181430 |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus B |
| Agent： | Human rhinovirus 17 |
| Strain： | 33342 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Human rhinovirus 17, complete genome |
| Comments： | ATCC®xa0VR-1663™ was treated with diethyl ether at ATCC® to remove Mycoplasma. The previous item number was ATCC®xa0VR-1127™ (NIAID Research Reference Reagent V-106-003-021). Strain 33342 is the prototype strain for Human rhinovirus 17. |
| Effect on Host： | CPE, rounding, sloughing |
| Recommended Host： | H1HeLa (ATCC® CRL-1958™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and then wash monolayer with PBS or serum free medium prior to inoculation. Inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb for 1 hour at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 3 days at 33°C in a humidified 5% CO2 atmosphere while rocking, until CPE is progressed throughout |
| Name of Depositor： | NIAID, ATCC |
| Source： | Presumed from throat swab from adult with upper respiratory illness, North Carolina, 1959 |
| References： | Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967. Tapparel C, et al. New complete genome sequences of human rhinoviruses shed light on their phylogeny and genomic features. BMC Genomics 8: 224, 2007. PubMed: 17623054 |
| Cross References： | Nucleotide (GenBank) : EF173420.1 Human rhinovirus 17, complete genome |
