| 产品名称： | pPCC41A2 41A2 |
|---|---|
| 商品货号： | TS181983 |
| Designations： | pPCC41A2 41A2 |
| GenBank Number： | M13573 |
| Species： | Homo sapiens, human |
| Depositors： | RA Gravel |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.350000023841858 Tissue: SV40-transformed fibroblast Gene product: propionyl Coenzyme A carboxylase, beta polypeptide( propionyl-CoA carboxylase, beta subunit, propionyl-CoA carboxylase, beta chain) PCCB Alleles: A2, C1, A1, B2, C2, B1 |
| Insert Size (kb)： | 1.350 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.3; HindIII--3.3, 1.05; EcoRI--uncut; XhoI--3.0, 1.3; PstI--2.2, 1.4, 0.77. Contains continuous open reading frame. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The C-allele polymorphism is in strong disequilibrium with the EcoRI (A allele) polymorphism. The B and C alleles are in equilibrium. There is a low background with a final wash at 65C in 0.1X SSC/0.1% SDS. Enzyme(s) not detecting polymorphism: XbaI, DraI, BglII, RsaI. |
| References： | Lamhonwah AM, et al. Isolation of cDNA clones coding for the alpha and beta chains of human propionyl-CoA carboxylase: chromosomal assignments and DNA polymorphisms associated with PCCA and PCCB genes. Proc. Natl. Acad. Sci. USA 83: 4864-4868, 1986. PubMed: 3460076 Lamhonwah AM, Gravel RA. Propionicacidemia: absence of alpha-chain mRNA in fibroblasts from patients of the PCCA complementation group. Am. J. Hum. Genet. 41: 1124-1131, 1987. PubMed: 3687944 Waye JS, et al. Two PstI RFLPs in the PCCB gene on the long arm of chromosome 3. Nucleic Acids Res. 16: 2362, 1988. PubMed: 2895916 Barbara Weiffenbach, personal communication |
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| 产品名称： | pPCC41A2 41A2 |
|---|---|
| 商品货号： | TS181983 |
| Designations： | pPCC41A2 41A2 |
| GenBank Number： | M13573 |
| Species： | Homo sapiens, human |
| Depositors： | RA Gravel |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.350000023841858 Tissue: SV40-transformed fibroblast Gene product: propionyl Coenzyme A carboxylase, beta polypeptide( propionyl-CoA carboxylase, beta subunit, propionyl-CoA carboxylase, beta chain) PCCB Alleles: A2, C1, A1, B2, C2, B1 |
| Insert Size (kb)： | 1.350 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.3; HindIII--3.3, 1.05; EcoRI--uncut; XhoI--3.0, 1.3; PstI--2.2, 1.4, 0.77. Contains continuous open reading frame. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The C-allele polymorphism is in strong disequilibrium with the EcoRI (A allele) polymorphism. The B and C alleles are in equilibrium. There is a low background with a final wash at 65C in 0.1X SSC/0.1% SDS. Enzyme(s) not detecting polymorphism: XbaI, DraI, BglII, RsaI. |
| References： | Lamhonwah AM, et al. Isolation of cDNA clones coding for the alpha and beta chains of human propionyl-CoA carboxylase: chromosomal assignments and DNA polymorphisms associated with PCCA and PCCB genes. Proc. Natl. Acad. Sci. USA 83: 4864-4868, 1986. PubMed: 3460076 Lamhonwah AM, Gravel RA. Propionicacidemia: absence of alpha-chain mRNA in fibroblasts from patients of the PCCA complementation group. Am. J. Hum. Genet. 41: 1124-1131, 1987. PubMed: 3687944 Waye JS, et al. Two PstI RFLPs in the PCCB gene on the long arm of chromosome 3. Nucleic Acids Res. 16: 2362, 1988. PubMed: 2895916 Barbara Weiffenbach, personal communication |
