| 产品名称： | Quantitative Synthetic Middle East respiratory syndrome coronavirus (MERS-CoV) RNA |
|---|---|
| 商品货号： | TS182141 |
| Agent： | Quantitative Synthetic Middle East respiratory syndrome coronavirus (MERS-CoV) RNA |
| Applications： | ATCC®xa0Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load and for other molecular biology applications. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen Specification range: 1 x 105 to 1 x 106 copies/µL 100 µL per vial with Biomatrica RNAstable |
| Storage Conditions： | -70°C or colder |
| Intended Use： | The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent. |
| Comments： | Manufactured under ISO 13485 guidance Preparation includes fragments from the ORF1ab, ORF5, upper envelope (upE), ORF8b, nucleocapsid (N) protein gene, and 3 UTR regions The following primers and probe can be used with this nucleic acid preparation Ref   Corman VM, et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill 17(39): pii=20285, 2012.:Forward primer: GCAACGCGCGATTCAGTT Reverse primer: GCCTCTACACGGGACCCATA Probe: CTCTTCACATAATCGCCCCGAGCTCG |
| Name of Depositor： | ATCC |
| Special Collection： | RNA |
| References： | Corman VM, et al. Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Euro Surveill 17(49): pii=20334, 2012. Corman VM, et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill 17(39): pii=20285, 2012. Corman VM, et al. Performance and clinical validation of the RealStar® MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA. J. Clin. Virol. 60(2): 168-171, 2014. PubMed: 24726679 Lu X, et al. Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus. J. Clin. Microbiol. 52(1): 67-75, 2013. PubMed: 24153118 World Health Organization. Laboratory Testing for Middle East Respiratory Syndrome Coronavirus - Interim Guidance (Revised). WHO/MERS/LAB/15.1, June 2015. |
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| 产品名称： | Quantitative Synthetic Middle East respiratory syndrome coronavirus (MERS-CoV) RNA |
|---|---|
| 商品货号： | TS182141 |
| Agent： | Quantitative Synthetic Middle East respiratory syndrome coronavirus (MERS-CoV) RNA |
| Applications： | ATCC®xa0Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load and for other molecular biology applications. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen Specification range: 1 x 105 to 1 x 106 copies/µL 100 µL per vial with Biomatrica RNAstable |
| Storage Conditions： | -70°C or colder |
| Intended Use： | The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent. |
| Comments： | Manufactured under ISO 13485 guidance Preparation includes fragments from the ORF1ab, ORF5, upper envelope (upE), ORF8b, nucleocapsid (N) protein gene, and 3 UTR regions The following primers and probe can be used with this nucleic acid preparation Ref Corman VM, et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill 17(39): pii=20285, 2012.:Forward primer: GCAACGCGCGATTCAGTT Reverse primer: GCCTCTACACGGGACCCATA Probe: CTCTTCACATAATCGCCCCGAGCTCG |
| Name of Depositor： | ATCC |
| Special Collection： | RNA |
| References： | Corman VM, et al. Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Euro Surveill 17(49): pii=20334, 2012. Corman VM, et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill 17(39): pii=20285, 2012. Corman VM, et al. Performance and clinical validation of the RealStar® MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA. J. Clin. Virol. 60(2): 168-171, 2014. PubMed: 24726679 Lu X, et al. Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus. J. Clin. Microbiol. 52(1): 67-75, 2013. PubMed: 24153118 World Health Organization. Laboratory Testing for Middle East Respiratory Syndrome Coronavirus - Interim Guidance (Revised). WHO/MERS/LAB/15.1, June 2015. |
