YEp363
YEp363
规格:
货期:
编号:TS182352
品牌:Testobio
| 产品名称： | YEp363 |
|---|---|
| 商品货号： | TS182352 |
| Designations： | YEp363 |
| Depositors： | CJ Lusty |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.3999996185302730 Vector: YEp363 (plasmid) Promoters: Promoter none Construction: pNM480, YEp351 Marker(s):LEU2,ampR Construct size (kb): 8.399999618530273 Features: insert detection: lacZ marker(s): ampR, LEU2 promoter: none replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1; HindIII-2. The EcoRI site is not unique. Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; SalI--8.4; PstI--8.4; EcoRI--4.25, 4.15. One of 3 promoter-cloning, YE type shuttle vectors (TS182352 - 37730) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multiple cloning sequence is: 5 GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915 |
| Shipped： | freeze-dried |
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| 产品名称： | YEp363 |
|---|---|
| 商品货号： | TS182352 |
| Designations： | YEp363 |
| Depositors： | CJ Lusty |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.3999996185302730 Vector: YEp363 (plasmid) Promoters: Promoter none Construction: pNM480, YEp351 Marker(s):LEU2,ampR Construct size (kb): 8.399999618530273 Features: insert detection: lacZ marker(s): ampR, LEU2 promoter: none replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1; HindIII-2. The EcoRI site is not unique. Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; SalI--8.4; PstI--8.4; EcoRI--4.25, 4.15. One of 3 promoter-cloning, YE type shuttle vectors (TS182352 - 37730) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multiple cloning sequence is: 5 GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915 |
| Shipped： | freeze-dried |
