| 产品名称： | cPFKL3.0 |
|---|---|
| 商品货号： | TS182509 |
| Designations： | cPFKL3.0 |
| GenBank Number： | X15573 |
| Species： | Homo sapiens, human |
| Depositors： | Y Groner |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.0 Tissue: liver Gene product: phosphofructokinase, liver type( human liver-type 1-phosphofructokinase, Phosphofructokinase, liver type) PFKL Alleles: A1, A2 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0 (doublet); BamHI--3.0, 2.0, 1.0; SalI--6.0; XhoI/PstI--3.0, 1.8, 0.55 (doublet); XbaI--3.0 (doublet). This was constructed from 3 overlapping cDNAs. When transcribed and translated in vitro, a polypeptide of 80 kDa recognized by anti-PFKL antibodies is generated. The clone detects the following genomic restriction fragments (kb), with those not from PFKL indicated by a *: BamHI--8.6, 7.5, 5.0, 4.2*, 3.9*, 3.3, 2.9, 2.2*; KpnI--14, 11, 4.2, 3.3*, 2.6; HindIII-- >20, 12.3, 5.2. A fragment specific to PFKL can be generated from 3 sequences. The insert contains the complete coding sequences, with 5 and 3 untranslated sequences. The KpnI polymorphic site is located in the 3.3 kb BamHI genomic fragment . |
| References： | Elson A, et al. The structure of the human liver-type phosphofructokinase gene. Genomics 7: 47-56, 1990. PubMed: 2139864 Levanon D, et al. The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK. DNA 8: 733-743, 1989. PubMed: 2533063 |
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| 产品名称： | cPFKL3.0 |
|---|---|
| 商品货号： | TS182509 |
| Designations： | cPFKL3.0 |
| GenBank Number： | X15573 |
| Species： | Homo sapiens, human |
| Depositors： | Y Groner |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.0 Tissue: liver Gene product: phosphofructokinase, liver type( human liver-type 1-phosphofructokinase, Phosphofructokinase, liver type) PFKL Alleles: A1, A2 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0 (doublet); BamHI--3.0, 2.0, 1.0; SalI--6.0; XhoI/PstI--3.0, 1.8, 0.55 (doublet); XbaI--3.0 (doublet). This was constructed from 3 overlapping cDNAs. When transcribed and translated in vitro, a polypeptide of 80 kDa recognized by anti-PFKL antibodies is generated. The clone detects the following genomic restriction fragments (kb), with those not from PFKL indicated by a *: BamHI--8.6, 7.5, 5.0, 4.2*, 3.9*, 3.3, 2.9, 2.2*; KpnI--14, 11, 4.2, 3.3*, 2.6; HindIII-- >20, 12.3, 5.2. A fragment specific to PFKL can be generated from 3 sequences. The insert contains the complete coding sequences, with 5 and 3 untranslated sequences. The KpnI polymorphic site is located in the 3.3 kb BamHI genomic fragment . |
| References： | Elson A, et al. The structure of the human liver-type phosphofructokinase gene. Genomics 7: 47-56, 1990. PubMed: 2139864 Levanon D, et al. The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK. DNA 8: 733-743, 1989. PubMed: 2533063 |
