| 产品名称： | pFH111 |
|---|---|
| 商品货号： | TS182619 |
| Designations： | pFH111 |
| GenBank Number： | X02761 |
| Species： | Homo sapiens, human |
| Depositors： | FE Baralle |
| Applications： | A 0.5 kb HindIII/BamHI fragment has been used to detect the TaqI polymorphism. |
| Vector： | Construct size (kb): 5.3 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.9 Tissue: Hs578T cell line Gene product: fibronectin 1 FN1 Alleles: A2, E2, A1, B2, D2, B1, D1, E1 |
| Insert Size (kb)： | 1.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--4.8, 0.6; HindIII--5.6; BamHI--4.5, 0.7; HindIII/BamHI--3.0, 1.5, 0.7; EcoRI--3.6, 1.5. A 0.5 kb HindIII/BamHI fragment has been used to detect the TaqI polymorphism. The 2.25/2.2 kb TaqI RFLP can be resolved on 1% agarose gels run for 20 cm. Enzyme(s) not detecting polymorphism: HaeIII, HindIII, EcoRI, PstI, BglII. |
| References： | Kornblihtt AR, et al. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene. EMBO J. 4: 1755-1759, 1985. PubMed: 2992939 Gardella R, et al. A new TaqI RFLP of the human fibronectin (FN1) gene. Nucleic Acids Res. 17: 10520, 1989. PubMed: 2574858 |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | pFH111 |
|---|---|
| 商品货号： | TS182619 |
| Designations： | pFH111 |
| GenBank Number： | X02761 |
| Species： | Homo sapiens, human |
| Depositors： | FE Baralle |
| Applications： | A 0.5 kb HindIII/BamHI fragment has been used to detect the TaqI polymorphism. |
| Vector： | Construct size (kb): 5.3 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.9 Tissue: Hs578T cell line Gene product: fibronectin 1 FN1 Alleles: A2, E2, A1, B2, D2, B1, D1, E1 |
| Insert Size (kb)： | 1.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--4.8, 0.6; HindIII--5.6; BamHI--4.5, 0.7; HindIII/BamHI--3.0, 1.5, 0.7; EcoRI--3.6, 1.5. A 0.5 kb HindIII/BamHI fragment has been used to detect the TaqI polymorphism. The 2.25/2.2 kb TaqI RFLP can be resolved on 1% agarose gels run for 20 cm. Enzyme(s) not detecting polymorphism: HaeIII, HindIII, EcoRI, PstI, BglII. |
| References： | Kornblihtt AR, et al. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene. EMBO J. 4: 1755-1759, 1985. PubMed: 2992939 Gardella R, et al. A new TaqI RFLP of the human fibronectin (FN1) gene. Nucleic Acids Res. 17: 10520, 1989. PubMed: 2574858 |
