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HHCME76
HHCME76
规格:
货期:
编号:TS182669
品牌:Testobio
产品名称: HHCME76
商品货号: TS182669
Designations: HHCME76
GenBank Number:

M78018

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ATP1A*
Gene name: ATPase, Na+K+, alpha polypeptide
Contains complete coding sequence?: U
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: M78018; EST: EST01604; Primer: M13 Reverse
Insert lengths(kb): 1.5
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205)
Gene product: ATPase, Na+K+, alpha polypeptide ATP1A*
Insert Size (kb): 1.5
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; BamHI--3.1, 1.4; PstI--3.4, 1.1.
EST01604 matches GenBank accession HUMATP1A2 with 97.73% identity over the sequence range: 140-316.
Sequenced from the M13 reverse primer.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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HHCME76

  • 货号: TS182669
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: HHCME76
商品货号: TS182669
Designations: HHCME76
GenBank Number:

M78018

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ATP1A*
Gene name: ATPase, Na+K+, alpha polypeptide
Contains complete coding sequence?: U
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: M78018; EST: EST01604; Primer: M13 Reverse
Insert lengths(kb): 1.5
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205)
Gene product: ATPase, Na+K+, alpha polypeptide ATP1A*
Insert Size (kb): 1.5
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; BamHI--3.1, 1.4; PstI--3.4, 1.1.
EST01604 matches GenBank accession HUMATP1A2 with 97.73% identity over the sequence range: 140-316.
Sequenced from the M13 reverse primer.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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