| 产品名称： | pHHH115.2 |
|---|---|
| 商品货号： | TS182983 |
| Designations： | pHHH115.2 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: DNA Segment, single copy D2S54 Alleles: M1, M2 |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 2.3. An EcoRI/HindIII double digest shows an insert fragment size of 2.3 kb. This plasmid shows some instability. We recommend growing on LB agar with ampicillin, and picking colonies to select the correct clone. Codominant segregation of the MspI polymorphism has been demonstrated in 45 3-generation families. Enzyme(s) not detecting polymorphism: RsaI, PstI, BglII, PvuII, TaqI. |
| References： | Hoff M, et al. Isolation and mapping of a polymorphic DNA sequence pHHH115.2 on chromosome 2 (D2S54). Nucleic Acids Res. 15: 10077, 1987. PubMed: 2892171 Yusuke Nakamura, personal communication |
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| 产品名称： | pHHH115.2 |
|---|---|
| 商品货号： | TS182983 |
| Designations： | pHHH115.2 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: DNA Segment, single copy D2S54 Alleles: M1, M2 |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 2.3. An EcoRI/HindIII double digest shows an insert fragment size of 2.3 kb. This plasmid shows some instability. We recommend growing on LB agar with ampicillin, and picking colonies to select the correct clone. Codominant segregation of the MspI polymorphism has been demonstrated in 45 3-generation families. Enzyme(s) not detecting polymorphism: RsaI, PstI, BglII, PvuII, TaqI. |
| References： | Hoff M, et al. Isolation and mapping of a polymorphic DNA sequence pHHH115.2 on chromosome 2 (D2S54). Nucleic Acids Res. 15: 10077, 1987. PubMed: 2892171 Yusuke Nakamura, personal communication |
