Crithidia ricardoi Sibajev et al.

Crithidia ricardoi Sibajev et al.

规格:

货期:

编号:TS183144

品牌:Testobio

产品名称：	Crithidia ricardoi Sibajev et al.
商品货号：	TS183144
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：	mosquito Isolation date: March 7, 1991
Product Format：	frozen
Storage Conditions：	Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic：	Axenic
Type Strain：	no
Medium：	ATCC^® Medium 355: Crithidia medium
Growth Conditions：	Temperature: 25°C
Cryopreservation：	Prepare a 10% (v/v) sterile DMSO solution in fresh ATCC Medium 355.xa0 Transfer a culture at peak density to centrifuge tubes and centrifuge at 525 x g for 5 minutes. Remove the supernatant and resuspend the cells in ATCC medium 355 to a concentration of 2 x 10⁶ to 2 x 10⁷ cells/ml. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 10⁶ and 10⁷ cells/ml and 5% (v/v) DMSO. Distribute the cell suspension in 0.5 ml aliquots into 1.0-2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).xa0 The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0 The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer. To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial. Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 ml of fresh ATCC medium 355 in a 16 x 125 mm screw-capped test tube. Incubate upright at 25°C with caps screwed on tightly.
Name of Depositor：	A Sibajev
Year of Origin：	March 7, 1991
References：	Sibajev A, et al. Crithidia ricardoi sp. n. a new species of Trypanosomatidae isolated from Culex saltanensis Dyar, 1928 (Diptera; Culicidae). Mem. Inst. Oswaldo Cruz Rio J. 88: 541-545, 1993. digestive tract of mosquito, Culex saltanensis, Rio de Janeiro, Brazil

首页 > 产品中心 > 微生物培养 > 菌株 > null > Crithidia ricardoi Sibajev et al.

最新活动福利

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

Crithidia ricardoi Sibajev et al.

货号: TS183144
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	Crithidia ricardoi Sibajev et al.
商品货号：	TS183144
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：	mosquito Isolation date: March 7, 1991
Product Format：	frozen
Storage Conditions：	Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic：	Axenic
Type Strain：	no
Medium：	ATCC^® Medium 355: Crithidia medium
Growth Conditions：	Temperature: 25°C
Cryopreservation：	Prepare a 10% (v/v) sterile DMSO solution in fresh ATCC Medium 355.xa0 Transfer a culture at peak density to centrifuge tubes and centrifuge at 525 x g for 5 minutes. Remove the supernatant and resuspend the cells in ATCC medium 355 to a concentration of 2 x 10⁶ to 2 x 10⁷ cells/ml. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 10⁶ and 10⁷ cells/ml and 5% (v/v) DMSO. Distribute the cell suspension in 0.5 ml aliquots into 1.0-2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).xa0 The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0 The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer. To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial. Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 ml of fresh ATCC medium 355 in a 16 x 125 mm screw-capped test tube. Incubate upright at 25°C with caps screwed on tightly.
Name of Depositor：	A Sibajev
Year of Origin：	March 7, 1991
References：	Sibajev A, et al. Crithidia ricardoi sp. n. a new species of Trypanosomatidae isolated from Culex saltanensis Dyar, 1928 (Diptera; Culicidae). Mem. Inst. Oswaldo Cruz Rio J. 88: 541-545, 1993. digestive tract of mosquito, Culex saltanensis, Rio de Janeiro, Brazil

testopic

小红书

扫码关注小红书账号

关注我们

联系电话：0574-87917803

章丽敏：17757489010

朱静：18067106081

李晴：18067408036

李晶晶：13375187189

周雪：13372109301

邮箱：testobio@163.com

地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼

在线留言

备案号：浙ICP备2021006467号-1

合作单位: