M158

规格:

货期:

编号:TS183175

品牌:Testobio

产品名称：	M158
商品货号：	TS183175
Organism：	Mus musculus, mouse
Tissue：	breast; mammary gland
Cell Type：	epithelial cell
Product Format：	frozen
Morphology：	epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	breast cancer
Applications：	The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene.
Clinical Data：	female
Genes Expressed：	Myc/c-Myc, expressed
Tumorigenic：	yes
Comments：	The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene.xa0 c-Myc plays a critical role in activating the transcription of growth-related genes.xa0 Elevated expression of c-Myc has been found in almost one-third of breast and colon carcinomas.xa0xa0 The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 8 X 10³ to 1.5 X 10⁴ viable cells/cm² is recommended. Incubate cultures at 37°C. Subculture when the cell concentration is between 1 X 10⁵ to 2 X 10⁵ cells/cm². Subcultivation ratio: A subcultivation ratio of 1:8 to 1:15 is recommended. Medium renewal: Every 2 to 3 days.
Cryopreservation：	Freeze medium: complete growth medium supplemented with an additional 10% bovine calf serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°Cxa0 Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor：	P Leder
References：	Stewart TA, et al. Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell 38: 627-637, 1984 PubMed: 6488314 Elson A, et al. Protein-tyrosine phosphatase epsilon. An isoform specifically expressed in mouse mammary tumors initiated by v-Ha-ras OR neu. J. Biol. Chem. 270(44): 26116-26122, 1995 PubMed: 7592814 Amundadottir LT, et al. Signal transduction pathways activated and required for mammary carcinogenesis in response to specific oncogenes. Oncogene 16(6): 737-746, 1998 PubMed:9488037 Fantin VR, et al. A novel mitochondriotoxic small molecule that selectively inhibits tumor cell growth. Cancer Cell 2(1): 29-42, 2002 PubMed: 12150823

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M158

货号: TS183175
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产品名称：	M158
商品货号：	TS183175
Organism：	Mus musculus, mouse
Tissue：	breast; mammary gland
Cell Type：	epithelial cell
Product Format：	frozen
Morphology：	epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	breast cancer
Applications：	The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene.
Clinical Data：	female
Genes Expressed：	Myc/c-Myc, expressed
Tumorigenic：	yes
Comments：	The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene.xa0 c-Myc plays a critical role in activating the transcription of growth-related genes.xa0 Elevated expression of c-Myc has been found in almost one-third of breast and colon carcinomas.xa0xa0 The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 8 X 10³ to 1.5 X 10⁴ viable cells/cm² is recommended. Incubate cultures at 37°C. Subculture when the cell concentration is between 1 X 10⁵ to 2 X 10⁵ cells/cm². Subcultivation ratio: A subcultivation ratio of 1:8 to 1:15 is recommended. Medium renewal: Every 2 to 3 days.
Cryopreservation：	Freeze medium: complete growth medium supplemented with an additional 10% bovine calf serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°Cxa0 Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor：	P Leder
References：	Stewart TA, et al. Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell 38: 627-637, 1984 PubMed: 6488314 Elson A, et al. Protein-tyrosine phosphatase epsilon. An isoform specifically expressed in mouse mammary tumors initiated by v-Ha-ras OR neu. J. Biol. Chem. 270(44): 26116-26122, 1995 PubMed: 7592814 Amundadottir LT, et al. Signal transduction pathways activated and required for mammary carcinogenesis in response to specific oncogenes. Oncogene 16(6): 737-746, 1998 PubMed:9488037 Fantin VR, et al. A novel mitochondriotoxic small molecule that selectively inhibits tumor cell growth. Cancer Cell 2(1): 29-42, 2002 PubMed: 12150823