pJA52
pJA52
规格:
货期:
编号:TS183284
品牌:Testobio
| 产品名称： | pJA52 |
|---|---|
| 商品货号： | TS183284 |
| Designations： | pJA52 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.8200001716613770 Vector: pJA52 (phagemid) Promoters: Promoter lac Construction: pUC Marker(s):TRP1,ampR,kanR Construct size (kb): 6.820000171661377 Features: insert detection: lacZ marker(s): ampR, kanR, TRP1 promoter: lac replicon: pMB1, f1 |
| Applications： | shuttle vector vector for other uses vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; PstI--4.6, 1.7, 0.6; HindIII--3.7, 1.6, 1.5; SmaI--3.7, 3.2; BglII--5.8, 1.0. Shuttle vector to facilitate construction of gene disruptions in E. coli. The gene of interest is cleaved at an appropriate site, made flush, and ligated to the SmaI fragment from this plasmid that contains kanR and the yeast selectable marker. The appropriate product is selected for by kanR in E. coli. The order of the major features in this plasmid is: TRP1 - kanR - EcoRI/MCS/HindIII - f1 ori - ampR - pMB1 ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Stephen J Elledge, personal communication |
| Related Products： | component of:ATCC 77255 |
| Shipped： | freeze-dried |
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| 产品名称： | pJA52 |
|---|---|
| 商品货号： | TS183284 |
| Designations： | pJA52 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.8200001716613770 Vector: pJA52 (phagemid) Promoters: Promoter lac Construction: pUC Marker(s):TRP1,ampR,kanR Construct size (kb): 6.820000171661377 Features: insert detection: lacZ marker(s): ampR, kanR, TRP1 promoter: lac replicon: pMB1, f1 |
| Applications： | shuttle vector vector for other uses vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; PstI--4.6, 1.7, 0.6; HindIII--3.7, 1.6, 1.5; SmaI--3.7, 3.2; BglII--5.8, 1.0. Shuttle vector to facilitate construction of gene disruptions in E. coli. The gene of interest is cleaved at an appropriate site, made flush, and ligated to the SmaI fragment from this plasmid that contains kanR and the yeast selectable marker. The appropriate product is selected for by kanR in E. coli. The order of the major features in this plasmid is: TRP1 - kanR - EcoRI/MCS/HindIII - f1 ori - ampR - pMB1 ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Stephen J Elledge, personal communication |
| Related Products： | component of:ATCC 77255 |
| Shipped： | freeze-dried |
