| 产品名称： | pSCH2009 |
|---|---|
| 商品货号： | TS183905 |
| Designations： | pSCH2009 |
| GenBank Number： | M21682 |
| Species： | Klebsiella pneumoniae (Schroeter) Trevisan |
| Depositors： | KJ Shaw |
| Applications： | Due to sequence similarity between aac(6)-Ib and aac(6)-IIa genes, the aac(6)-Ib probe is used to detect both genes. |
| Vector： | Construct size (kb): 3.299999952316284 |
| Insert： | DNA: cDNA Insert information: Insert size (kb): 0.331 Genexa0: aminoglycoside 6x92-N-acetyltransferase Gene symbolxa0: aac(6x92)-Ib Sourcexa0: xa0Klebsiella pneumoniae Insert endxa0: AvaI Insert endxa0: DdeI Genbank accessionxa0: M21682 xa0xa0xa0xa0xa0xa0xa0xa0xa0 Nucleotides 1-331 of the insert correspond to nucleotides xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 471 - 801 of the Genbank sequence M21682. Insert lengths(kb): 0.3310000002384186 Gene product: aminoglycoside 6-N-acetyltransferase aac(6)-Ib |
| Insert Size (kb)： | 0.331 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | xa0Freeze dried E. coli containing the phagemid |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/XhoI--3.0, 0.4; HindIII--3.4; XhoI--3.4. The insert contains the following restriction sites (approximate kb from the 5 end): ClaI--0.04; ScaI--0.11; SacI--0.32. A single gel purification of the PCR generated probe is necessary since flanking regions will co-amplify with the gene specific sequence. Failure to do so often results in high backgrounds and false positives with clinical E. coli strains. Due to sequence similarity between aac(6)-Ib and aac(6)-IIa genes, the aac(6)-Ib probe is used to detect both genes. A hybridization probe may be generated using the following vector specific PCR primers: modified T3 = 5-CCCCTCACTAAAGGGAACAAAAGCTG-3 and modified T7 = 5-CGCGTAATACGACTCACTATAGGGCGAA-3. |
| References： | Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996 |
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| 产品名称： | pSCH2009 |
|---|---|
| 商品货号： | TS183905 |
| Designations： | pSCH2009 |
| GenBank Number： | M21682 |
| Species： | Klebsiella pneumoniae (Schroeter) Trevisan |
| Depositors： | KJ Shaw |
| Applications： | Due to sequence similarity between aac(6)-Ib and aac(6)-IIa genes, the aac(6)-Ib probe is used to detect both genes. |
| Vector： | Construct size (kb): 3.299999952316284 |
| Insert： | DNA: cDNA Insert information: Insert size (kb): 0.331 Genexa0: aminoglycoside 6x92-N-acetyltransferase Gene symbolxa0: aac(6x92)-Ib Sourcexa0: xa0Klebsiella pneumoniae Insert endxa0: AvaI Insert endxa0: DdeI Genbank accessionxa0: M21682 xa0xa0xa0xa0xa0xa0xa0xa0xa0 Nucleotides 1-331 of the insert correspond to nucleotides xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 471 - 801 of the Genbank sequence M21682. Insert lengths(kb): 0.3310000002384186 Gene product: aminoglycoside 6-N-acetyltransferase aac(6)-Ib |
| Insert Size (kb)： | 0.331 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | xa0Freeze dried E. coli containing the phagemid |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/XhoI--3.0, 0.4; HindIII--3.4; XhoI--3.4. The insert contains the following restriction sites (approximate kb from the 5 end): ClaI--0.04; ScaI--0.11; SacI--0.32. A single gel purification of the PCR generated probe is necessary since flanking regions will co-amplify with the gene specific sequence. Failure to do so often results in high backgrounds and false positives with clinical E. coli strains. Due to sequence similarity between aac(6)-Ib and aac(6)-IIa genes, the aac(6)-Ib probe is used to detect both genes. A hybridization probe may be generated using the following vector specific PCR primers: modified T3 = 5-CCCCTCACTAAAGGGAACAAAAGCTG-3 and modified T7 = 5-CGCGTAATACGACTCACTATAGGGCGAA-3. |
| References： | Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996 |
