| 产品名称： | Acanthamoeba castellanii (Douglas) Page |
|---|---|
| 商品货号： | TS184355 |
| Strain Designations： | 40AB |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | clinical specimen - animal Vienna Austria Isolation date: 1998 |
| Product Format： | frozen |
| Storage Conditions： | liquid nitrogen vapor phase |
| Type Strain： | no |
| Medium： | ATCC® Medium 712: PYG w/ Additives |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days. Interval: every month |
| Subcultivation： | Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days. Interval: every month |
| Cryopreservation： | Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor： | J Walochnik |
| Chain of Custody： | J Walochnik |
| Year of Origin： | 1998 |
| References： | Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891 necrotic tissue of a Basiliscus plumifrons (green basilisk) Walochnik J, et al. Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basilliscus plumifrons (Sauria: Iguanidae). Parasitol. Res. 85: 601, 1999. PubMed: 10382612 |
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| 产品名称： | Acanthamoeba castellanii (Douglas) Page |
|---|---|
| 商品货号： | TS184355 |
| Strain Designations： | 40AB |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | clinical specimen - animal Vienna Austria Isolation date: 1998 |
| Product Format： | frozen |
| Storage Conditions： | liquid nitrogen vapor phase |
| Type Strain： | no |
| Medium： | ATCC® Medium 712: PYG w/ Additives |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days. Interval: every month |
| Subcultivation： | Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days. Interval: every month |
| Cryopreservation： | Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor： | J Walochnik |
| Chain of Custody： | J Walochnik |
| Year of Origin： | 1998 |
| References： | Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891 necrotic tissue of a Basiliscus plumifrons (green basilisk) Walochnik J, et al. Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basilliscus plumifrons (Sauria: Iguanidae). Parasitol. Res. 85: 601, 1999. PubMed: 10382612 |
