| 产品名称： | F814 |
|---|---|
| 商品货号： | TS184483 |
| Designations： | F814 |
| Species： | Homo sapiens, human |
| Depositors： | J Mandel |
| Applications： | This probe can be used routinely to detect both the F8C and DXS52 (VNTR) RFLPs on 0.9 - 1.0 agarose gels. |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) F8C Alleles: F3, E1, E1, E2, F1, F2, B1, F1, F2, A1, A2, B2 |
| Insert Size (kb)： | 1.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.2; EcoRI/HindIII--2.7, 1.5; HindIII--2.7, 1.5; PstI--3.5, 0.68; XbaI--4.2. Linkage has been demonstrated between X-linked recessive hydrocephalus (HSAS) and DXS52 (theta = 0.03, LOD score = 6.52). Linkage has also been demonstrated between HSAS and F8 (theta = 0.00, LOD score = 4.32). This is a hybrid probe containing a fragment of the factor VIII gene that detects the BclI RFLP and a fragment close to the St14 minisatellite that detects the VNTR in several restriction digests including TaqI, BclI, and MspI. This probe can be used routinely to detect both the F8C and DXS52 (VNTR) RFLPs on 0.9 - 1.0 agarose gels. It also gives a clear pattern for DXS52 on TaqI blots. This probe does not detect the 2-allele RFLPs of St14-1 (ATCC 61372) (TaqI, BclI). |
| References： | Mandel JL, et al. Genetic mapping of the human X chromosome: linkage analysis of the q26-q28 region that includes the fragile X locus and isolation of expressed sequences. Cold Spring Harbor Symp. Quant. Biol. 51: 195-203, 1986. PubMed: 3472716 Heilig R, et al. Improved DNA markers for efficient analysis of fragile X families. Am. J. Med. Genet. 30: 543-550, 1988. PubMed: 2902795 Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147 Willems PJ, et al. Assignment of X-linked hydrocephalus to Xq28 by linkage analysis. Genomics 8: 367-370, 1990. PubMed: 1979056 Jean Louis Mandel, personal communication |
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| 产品名称： | F814 |
|---|---|
| 商品货号： | TS184483 |
| Designations： | F814 |
| Species： | Homo sapiens, human |
| Depositors： | J Mandel |
| Applications： | This probe can be used routinely to detect both the F8C and DXS52 (VNTR) RFLPs on 0.9 - 1.0 agarose gels. |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) F8C Alleles: F3, E1, E1, E2, F1, F2, B1, F1, F2, A1, A2, B2 |
| Insert Size (kb)： | 1.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.2; EcoRI/HindIII--2.7, 1.5; HindIII--2.7, 1.5; PstI--3.5, 0.68; XbaI--4.2. Linkage has been demonstrated between X-linked recessive hydrocephalus (HSAS) and DXS52 (theta = 0.03, LOD score = 6.52). Linkage has also been demonstrated between HSAS and F8 (theta = 0.00, LOD score = 4.32). This is a hybrid probe containing a fragment of the factor VIII gene that detects the BclI RFLP and a fragment close to the St14 minisatellite that detects the VNTR in several restriction digests including TaqI, BclI, and MspI. This probe can be used routinely to detect both the F8C and DXS52 (VNTR) RFLPs on 0.9 - 1.0 agarose gels. It also gives a clear pattern for DXS52 on TaqI blots. This probe does not detect the 2-allele RFLPs of St14-1 (ATCC 61372) (TaqI, BclI). |
| References： | Mandel JL, et al. Genetic mapping of the human X chromosome: linkage analysis of the q26-q28 region that includes the fragile X locus and isolation of expressed sequences. Cold Spring Harbor Symp. Quant. Biol. 51: 195-203, 1986. PubMed: 3472716 Heilig R, et al. Improved DNA markers for efficient analysis of fragile X families. Am. J. Med. Genet. 30: 543-550, 1988. PubMed: 2902795 Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147 Willems PJ, et al. Assignment of X-linked hydrocephalus to Xq28 by linkage analysis. Genomics 8: 367-370, 1990. PubMed: 1979056 Jean Louis Mandel, personal communication |
