| 产品名称： | cDMD 9-14 63-1, cDMD9-14 |
|---|---|
| 商品货号： | TS184658 |
| Designations： | cDMD 9-14 63-1, cDMD9-14 |
| GenBank Number： | M18533 |
| Species： | Homo sapiens, human |
| Applications： | For plasmid amplification, use a lac Iq host. |
| Vector： | Construct size (kb): 9.100000381469727 |
| Insert： | DNA: cDNA Insert lengths(kb): 6.099999904632568 Tissue: fetal muscle Gene product: dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272 DMD Alleles: U2, A1, A2, C1, T1, U1, A1, A1, A2, A2, B1, B2, C2, D2, R1, R2, T1, T2, T2, T3, D1 |
| Insert Size (kb)： | 6.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.1, 3.0. For plasmid amplification, use a lac Iq host. Inserted into EcoRI site with 5 end closer to T7 promoter. Contains internal HindIII (3), PstI (4), BamHI (2), HincII (3), BglII (2), and XbaI sites. Codominant inheritance of the MspI RFLP demonstrated in 3 two-generation families. ATCC 57676 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not required for ATCC 57676, but is for TS184658. Includes probes 9-14. A BamHI digest gives probes 9, 10, and 11-14. Detects genomic HindIII fragments of 7.8, 1, 8.3, 2.3, 1, 8.8, 6, 3.5, 2.55, 2.8, 6.6, 12, 2.4, 1.45, 1.5, 1.9, 2.1, 5.2, 6.8, 10, 1.8, 5.9, 7.8, and 6 kb. Of the DMD cDNA clones, 1-2a includes nucleotides 1-1538; 2b-3: 1455 to approximately 2600; 4-5a: 2600-4550; 5b-7: 4400-6900; 8: 6900-7800; 9-14: 7800-13900 (in base pairs from the 5 end of the cDNA). Enzyme(s) not detecting polymorphism: HindIII. |
| References： | Lindlof M, et al. Gene deletions in X-linked muscular dystrophy. Am. J. Hum. Genet. 44: 496-503, 1989. PubMed: 2929594 Wagner M, et al. MspI RFLP for Duchenne muscular dystrophy cDNA subclone 9. Nucleic Acids Res. 17: 3328, 1989. PubMed: 2471152 Den Dunnen JT, et al. Topography of the Duchenne muscular dystrophy (DMD) gene: FIGE and cDNA analysis of 194 cases reveals 115 deletions and 13 duplications. Am. J. Hum. Genet. 45: 835-847, 1989. PubMed: 2573997 Lucotte G, et al. Molecular deletion patterns in Duchenne muscular dystrophy patients. Ann. Genet. 32: 214-219, 1989. PubMed: 2610487 Liechti-Gallati S, et al. RFLPs for Duchenne muscular dystrophy cDNA clones 9 and 10. Am. J. Hum. Genet. 46: 1090-1094, 1990. PubMed: 1971151 Dominguez-Steglich M, et al. The dystrophin gene is autosomally located on a microchromosome in chicken. Genomics 8: 536-540, 1990. PubMed: 2286374 Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877 Koenig M, et al. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein. Cell 53: 219-228, 1988. PubMed: 3282674 Louis M Kunkel, personal communication |
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| 产品名称： | cDMD 9-14 63-1, cDMD9-14 |
|---|---|
| 商品货号： | TS184658 |
| Designations： | cDMD 9-14 63-1, cDMD9-14 |
| GenBank Number： | M18533 |
| Species： | Homo sapiens, human |
| Applications： | For plasmid amplification, use a lac Iq host. |
| Vector： | Construct size (kb): 9.100000381469727 |
| Insert： | DNA: cDNA Insert lengths(kb): 6.099999904632568 Tissue: fetal muscle Gene product: dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272 DMD Alleles: U2, A1, A2, C1, T1, U1, A1, A1, A2, A2, B1, B2, C2, D2, R1, R2, T1, T2, T2, T3, D1 |
| Insert Size (kb)： | 6.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.1, 3.0. For plasmid amplification, use a lac Iq host. Inserted into EcoRI site with 5 end closer to T7 promoter. Contains internal HindIII (3), PstI (4), BamHI (2), HincII (3), BglII (2), and XbaI sites. Codominant inheritance of the MspI RFLP demonstrated in 3 two-generation families. ATCC 57676 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not required for ATCC 57676, but is for TS184658. Includes probes 9-14. A BamHI digest gives probes 9, 10, and 11-14. Detects genomic HindIII fragments of 7.8, 1, 8.3, 2.3, 1, 8.8, 6, 3.5, 2.55, 2.8, 6.6, 12, 2.4, 1.45, 1.5, 1.9, 2.1, 5.2, 6.8, 10, 1.8, 5.9, 7.8, and 6 kb. Of the DMD cDNA clones, 1-2a includes nucleotides 1-1538; 2b-3: 1455 to approximately 2600; 4-5a: 2600-4550; 5b-7: 4400-6900; 8: 6900-7800; 9-14: 7800-13900 (in base pairs from the 5 end of the cDNA). Enzyme(s) not detecting polymorphism: HindIII. |
| References： | Lindlof M, et al. Gene deletions in X-linked muscular dystrophy. Am. J. Hum. Genet. 44: 496-503, 1989. PubMed: 2929594 Wagner M, et al. MspI RFLP for Duchenne muscular dystrophy cDNA subclone 9. Nucleic Acids Res. 17: 3328, 1989. PubMed: 2471152 Den Dunnen JT, et al. Topography of the Duchenne muscular dystrophy (DMD) gene: FIGE and cDNA analysis of 194 cases reveals 115 deletions and 13 duplications. Am. J. Hum. Genet. 45: 835-847, 1989. PubMed: 2573997 Lucotte G, et al. Molecular deletion patterns in Duchenne muscular dystrophy patients. Ann. Genet. 32: 214-219, 1989. PubMed: 2610487 Liechti-Gallati S, et al. RFLPs for Duchenne muscular dystrophy cDNA clones 9 and 10. Am. J. Hum. Genet. 46: 1090-1094, 1990. PubMed: 1971151 Dominguez-Steglich M, et al. The dystrophin gene is autosomally located on a microchromosome in chicken. Genomics 8: 536-540, 1990. PubMed: 2286374 Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877 Koenig M, et al. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein. Cell 53: 219-228, 1988. PubMed: 3282674 Louis M Kunkel, personal communication |
