| 产品名称： | pUC18 |
|---|---|
| 商品货号： | TS184992 |
| Designations： | pUC18 |
| Depositors： | J Messing |
| Other IDs： | Nucleotide (GenBank) : VB0032 pUC18c cloning vector (beta-galactosidase mRNA on complementary strand). Nucleotide (GenBank) : VB0025 pUC18 cloning vector. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.686 Vector: pUC18 (plasmid) Promoters: Promoter lac Construction: pUC71K Marker(s):ampR Construct size (kb): 2.686 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 MCS: HindIII...EcoRI |
| Applications： | expression vector vector permitting construction of fusion proteins |
| Comments： | Expression vector encoding the beta-galactosidase alpha peptide (lacZ), permitting blue-white visual detection of re combinants. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879 Norrander J, et al. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26: 101-106, 1983. PubMed: 6323249 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried plasmid in E. coli |
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| 产品名称： | pUC18 |
|---|---|
| 商品货号： | TS184992 |
| Designations： | pUC18 |
| Depositors： | J Messing |
| Other IDs： | Nucleotide (GenBank) : VB0032 pUC18c cloning vector (beta-galactosidase mRNA on complementary strand). Nucleotide (GenBank) : VB0025 pUC18 cloning vector. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.686 Vector: pUC18 (plasmid) Promoters: Promoter lac Construction: pUC71K Marker(s):ampR Construct size (kb): 2.686 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 MCS: HindIII...EcoRI |
| Applications： | expression vector vector permitting construction of fusion proteins |
| Comments： | Expression vector encoding the beta-galactosidase alpha peptide (lacZ), permitting blue-white visual detection of re combinants. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879 Norrander J, et al. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26: 101-106, 1983. PubMed: 6323249 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried plasmid in E. coli |
