| 产品名称： | SU-DHL-16 |
|---|---|
| 商品货号： | TS185168 |
| Organism： | Homo sapiens, human |
| Tissue： | lymph node; Derived from Metastatic Site: peritoneal effusion |
| Product Format： | frozen |
| Morphology： | lymphoblast-like |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | large cell lymphoma; B cell lymphoma |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | t(14;18) chromosomal translocation, positive |
| Images： |  |
| Genes Expressed： | methionine aminopeptidase 2 (MetAP2), expressed cathelicidin antimicrobial peptide (CAMP) expression is induced upon exposure to 1,25(OH)2D3 bcl-6 protein, expressed |
| Comments： | The depositor states that this cell line is negative for the presence of Epstein-Barr virus(EBV) genome. ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. The SU-DHL-16 cell line possesses a t(14;18)(q32;q21) translocation. The SU-DHL-16 cell line contains a minor bcl-2 rearrangement. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium. An inoculum of 1 X 105 to 2 x 105 cells/mL is recommended. Subculture when cell concentration is between 1 x 106 and 2 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended. Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 10 |
| Name of Depositor： | A Epstein |
| Year of Origin： | 1980 |
| References： | Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039 |
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| 产品名称： | SU-DHL-16 |
|---|---|
| 商品货号： | TS185168 |
| Organism： | Homo sapiens, human |
| Tissue： | lymph node; Derived from Metastatic Site: peritoneal effusion |
| Product Format： | frozen |
| Morphology： | lymphoblast-like |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | large cell lymphoma; B cell lymphoma |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | t(14;18) chromosomal translocation, positive |
| Images： | |
| Genes Expressed： | methionine aminopeptidase 2 (MetAP2), expressed cathelicidin antimicrobial peptide (CAMP) expression is induced upon exposure to 1,25(OH)2D3 bcl-6 protein, expressed |
| Comments： | The depositor states that this cell line is negative for the presence of Epstein-Barr virus(EBV) genome. ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. The SU-DHL-16 cell line possesses a t(14;18)(q32;q21) translocation. The SU-DHL-16 cell line contains a minor bcl-2 rearrangement. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium. An inoculum of 1 X 105 to 2 x 105 cells/mL is recommended. Subculture when cell concentration is between 1 x 106 and 2 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended. Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 10 |
| Name of Depositor： | A Epstein |
| Year of Origin： | 1980 |
| References： | Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039 |
