pHKHK1-2
pHKHK1-2
规格:
货期:
编号:TS185250
品牌:Testobio
| 产品名称： | pHKHK1-2 |
|---|---|
| 商品货号： | TS185250 |
| Designations： | pHKHK1-2 |
| GenBank Number： | X78677 |
| Species： | Homo sapiens, human |
| Applications： | The first polyadenylation signal was used. |
| Vector： | Construct size (kb): 5.429999828338623 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCDM8 Intact vector size: 4.356 Type of vector: phagemid Vector end: BstXI Vector end: BstXI Cloning sites: HindIII XbaI XhoI BstXI PstI NotI Polylinker sites: HindIII XbaI XhoI BstXI --stuffer--BstXI XhoI PstI NotI XbaI Construction: CMV, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): replicon: pMB1, 1-587 replicon: M13, marker(s): supF, promoter: CMV, ->, 1516-2170 promoter for in vitro transcription: T7, ->, 2171-2189 MCS: HindIII...XbaI, ->, 2198-2655 terminator: SV40 splice site and polyadenylation, ->, 2656-3352 replicon: polyoma, 3353-4188 replicon: SV40, 4189-4352 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: KHK Gene name: ketohexokinase (fructokinase) Contains complete coding sequence?: N Tissue: Hep G2 cell line Disease: hepatocellular carcinoma Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.931 Cross references: DNA Seq. Acc.: X78677 Nucleotides 1-931 of the insert correspond to nucleotides 1-931 of X78677. Insert lengths(kb): 0.9309999942779541 Tissue: Hep G2 cell line hepatocellular carcinoma Disease: hepatocellular carcinoma Gene product: ketohexokinase (fructokinase) KHK |
| Insert Size (kb)： | 0.931 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--4.2, 1.2; HindIII--5.4; BamHI--3.8, 1.6; PstI--4.5, 0.5, 0.4; SacI--4.8, 0.5. Derived from the form C variant transcript (alternately spliced third coding exon). The first polyadenylation signal was used. |
| References： | Bonthron DT, et al. Molecular basis of essential fructosuria: molecular cloning and mutational analysis of human ketohexokinase (fructokinase). Hum. Mol. Genet. 3: 1627-1631, 1994. PubMed: 7833921 |
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| 产品名称： | pHKHK1-2 |
|---|---|
| 商品货号： | TS185250 |
| Designations： | pHKHK1-2 |
| GenBank Number： | X78677 |
| Species： | Homo sapiens, human |
| Applications： | The first polyadenylation signal was used. |
| Vector： | Construct size (kb): 5.429999828338623 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCDM8 Intact vector size: 4.356 Type of vector: phagemid Vector end: BstXI Vector end: BstXI Cloning sites: HindIII XbaI XhoI BstXI PstI NotI Polylinker sites: HindIII XbaI XhoI BstXI --stuffer--BstXI XhoI PstI NotI XbaI Construction: CMV, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): replicon: pMB1, 1-587 replicon: M13, marker(s): supF, promoter: CMV, ->, 1516-2170 promoter for in vitro transcription: T7, ->, 2171-2189 MCS: HindIII...XbaI, ->, 2198-2655 terminator: SV40 splice site and polyadenylation, ->, 2656-3352 replicon: polyoma, 3353-4188 replicon: SV40, 4189-4352 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: KHK Gene name: ketohexokinase (fructokinase) Contains complete coding sequence?: N Tissue: Hep G2 cell line Disease: hepatocellular carcinoma Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.931 Cross references: DNA Seq. Acc.: X78677 Nucleotides 1-931 of the insert correspond to nucleotides 1-931 of X78677. Insert lengths(kb): 0.9309999942779541 Tissue: Hep G2 cell line hepatocellular carcinoma Disease: hepatocellular carcinoma Gene product: ketohexokinase (fructokinase) KHK |
| Insert Size (kb)： | 0.931 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--4.2, 1.2; HindIII--5.4; BamHI--3.8, 1.6; PstI--4.5, 0.5, 0.4; SacI--4.8, 0.5. Derived from the form C variant transcript (alternately spliced third coding exon). The first polyadenylation signal was used. |
| References： | Bonthron DT, et al. Molecular basis of essential fructosuria: molecular cloning and mutational analysis of human ketohexokinase (fructokinase). Hum. Mol. Genet. 3: 1627-1631, 1994. PubMed: 7833921 |
