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mp40
mp40
规格:
货期:
编号:TS185498
品牌:Testobio
产品名称: mp40
商品货号: TS185498
Designations: mp40
GenBank Number:

M86671

Species: Mus musculus, mouse
Depositors: U Gubler
Vector:
Construct size (kb): 4.013999938964844
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: HindIII
Vector end: EcoRI
Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: Il12b
Genomic copy number: unique
Gene name: interleukin 12B (natural killer cell stimulatory factor 2,
cytotoxic lymphocyte maturation factor 2, p40)
Contains complete coding sequence?: Y
Chromosome: 11
Strain: C57BL/6
Tissue: spleen
Type of DNA: cDNA
Insert 5 end: HindIII
Insert 3 end: EcoRI
Insert size (kb): 1.05
Cross references: DNA Seq. Acc.: M86671
Insert lengths(kb): 1.049999952316284
Tissue: spleen
Gene product: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) Il12b
Insert Size (kb): 1.050
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 1.1; BamHI--4.3; XbaI--3.2, 0.84, 0.3.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.55, 1.02; AvaI--0.52; PvuII--0.79.
The sequence surrounding the initiation codon was modified to 5-ACCAUGG-3 (Kozak consensus) to optimize translation. Thus the sequence between the vector insertion site and the initiating ATG is not derived from the mRNA.
Classification: Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References:

Schoenhaut DS, et al. Cloning and expression of murine IL-12. J. Immunol. 148: 3433-3440, 1992. PubMed: 1350290

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mp40

  • 货号: TS185498
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: mp40
商品货号: TS185498
Designations: mp40
GenBank Number:

M86671

Species: Mus musculus, mouse
Depositors: U Gubler
Vector:
Construct size (kb): 4.013999938964844
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: HindIII
Vector end: EcoRI
Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: Il12b
Genomic copy number: unique
Gene name: interleukin 12B (natural killer cell stimulatory factor 2,
cytotoxic lymphocyte maturation factor 2, p40)
Contains complete coding sequence?: Y
Chromosome: 11
Strain: C57BL/6
Tissue: spleen
Type of DNA: cDNA
Insert 5 end: HindIII
Insert 3 end: EcoRI
Insert size (kb): 1.05
Cross references: DNA Seq. Acc.: M86671
Insert lengths(kb): 1.049999952316284
Tissue: spleen
Gene product: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) Il12b
Insert Size (kb): 1.050
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 1.1; BamHI--4.3; XbaI--3.2, 0.84, 0.3.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.55, 1.02; AvaI--0.52; PvuII--0.79.
The sequence surrounding the initiation codon was modified to 5-ACCAUGG-3 (Kozak consensus) to optimize translation. Thus the sequence between the vector insertion site and the initiating ATG is not derived from the mRNA.
Classification: Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References:

Schoenhaut DS, et al. Cloning and expression of murine IL-12. J. Immunol. 148: 3433-3440, 1992. PubMed: 1350290

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