| 产品名称： | pVE3 MB4597 |
|---|---|
| 商品货号： | TS185513 |
| Designations： | pVE3 MB4597 |
| Depositors： | Merck & Co., Inc., T MacNeil, Merck & Co., Inc. |
| U.S. Patent： | |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli RR1; K-12 RR1 |
| Vector Information： | Size (kb): 17.2999897003173800 Vector: pVE3 (plasmid) Construction: pVE1, pIJ39 Marker(s):ampR,tsrR Construct size (kb): 17.29998970031738 Features: marker(s): ampR, tsrR replicon: pVE1, pMB1 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--10.1, 5.9; HindIII--10.1, 5.9; BamHI--10.5, 2.8, 1.7. A shuttle vector capable of replication in Escherichia coli and several Streptomyces species including lividans, avermitilis, and venezuelae. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | MacNeil T, Gibbons PH. rDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes. US Patent 4,703,009 dated Oct 27 1987 MacNeil DJ. Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J. Bacteriol. 170: 5607-5612, 1988. PubMed: 3056907 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | pVE3 MB4597 |
|---|---|
| 商品货号： | TS185513 |
| Designations： | pVE3 MB4597 |
| Depositors： | Merck & Co., Inc., T MacNeil, Merck & Co., Inc. |
| U.S. Patent： | |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli RR1; K-12 RR1 |
| Vector Information： | Size (kb): 17.2999897003173800 Vector: pVE3 (plasmid) Construction: pVE1, pIJ39 Marker(s):ampR,tsrR Construct size (kb): 17.29998970031738 Features: marker(s): ampR, tsrR replicon: pVE1, pMB1 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--10.1, 5.9; HindIII--10.1, 5.9; BamHI--10.5, 2.8, 1.7. A shuttle vector capable of replication in Escherichia coli and several Streptomyces species including lividans, avermitilis, and venezuelae. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | MacNeil T, Gibbons PH. rDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes. US Patent 4,703,009 dated Oct 27 1987 MacNeil DJ. Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J. Bacteriol. 170: 5607-5612, 1988. PubMed: 3056907 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
