| 产品名称： | pKSK056 KS56 |
|---|---|
| 商品货号： | TS185764 |
| Designations： | pKSK056 KS56 |
| GenBank Number： | X05199 |
| Species： | Homo sapiens, human |
| Depositors： | University of Oklahoma Health Sciences Center, J Feretti, University of Oklahoma Health Sciences Center |
| Applications： | Encodes a plasminogen-streptokinase fusion protein expressed under control of lacPO. The fusion protein is extended at the N-terminus by the N-terminal hexapeptide of LacZ followed by four polylinker-encoded amino acids. |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: genomic Insert lengths(kb): 1.76199996471405 Gene product: streptokinase PLG |
| Insert Size (kb)： | 1.762 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 1.2, 0.9; BamHI--4.8; PstI--2.9, 1.9. The plasminogen portion corresponds to nt 196-697 of the reported sequence and encodes the K1 kringle domain and part of the K2 kringle domain. Downstream of the plasminogen sequence is a vector sequence encoding the peptide GGSSR and a truncated streptokinase gene lacking regulatory sequences and codons 1-39 but retaining the remainder of the coding sequence and the transcription terminator. Contains 2 internal PstI sites. Encodes a plasminogen-streptokinase fusion protein expressed under control of lacPO. The fusion protein is extended at the N-terminus by the N-terminal hexapeptide of LacZ followed by four polylinker-encoded amino acids. The plasminogen sequence was cloned into the SmaI site and the streptokinase sequence was cloned into the HincII site. |
| References： | Malke H, Ferretti JJExpression and properties of hybrid streptokinases extended by N-terminal plasminogen kringle domainsIn: Malke H, Ferretti JJGenetics and molecular biology of streptococci, lactococci, and enterococciWashington, DCAmerican Society for Microbiologypp. 184-189, 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pKSK056 KS56 |
|---|---|
| 商品货号： | TS185764 |
| Designations： | pKSK056 KS56 |
| GenBank Number： | X05199 |
| Species： | Homo sapiens, human |
| Depositors： | University of Oklahoma Health Sciences Center, J Feretti, University of Oklahoma Health Sciences Center |
| Applications： | Encodes a plasminogen-streptokinase fusion protein expressed under control of lacPO. The fusion protein is extended at the N-terminus by the N-terminal hexapeptide of LacZ followed by four polylinker-encoded amino acids. |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: genomic Insert lengths(kb): 1.76199996471405 Gene product: streptokinase PLG |
| Insert Size (kb)： | 1.762 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 1.2, 0.9; BamHI--4.8; PstI--2.9, 1.9. The plasminogen portion corresponds to nt 196-697 of the reported sequence and encodes the K1 kringle domain and part of the K2 kringle domain. Downstream of the plasminogen sequence is a vector sequence encoding the peptide GGSSR and a truncated streptokinase gene lacking regulatory sequences and codons 1-39 but retaining the remainder of the coding sequence and the transcription terminator. Contains 2 internal PstI sites. Encodes a plasminogen-streptokinase fusion protein expressed under control of lacPO. The fusion protein is extended at the N-terminus by the N-terminal hexapeptide of LacZ followed by four polylinker-encoded amino acids. The plasminogen sequence was cloned into the SmaI site and the streptokinase sequence was cloned into the HincII site. |
| References： | Malke H, Ferretti JJExpression and properties of hybrid streptokinases extended by N-terminal plasminogen kringle domainsIn: Malke H, Ferretti JJGenetics and molecular biology of streptococci, lactococci, and enterococciWashington, DCAmerican Society for Microbiologypp. 184-189, 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
