Biomedical Research and Development Material

Emerging infectious disease research

Negative control in differential gene expression studies

Produces laccase

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Yes

Multigene phylogeny and phenotypic characterization.xa0

Facultative intracellular pathogenxa0

Genome sequencing strain (Broad Institute, USA)

Designated as type strain of Cryptococcus neoformans (Sanfelice) Vuillemin by Hagen et al. (2015)

Temperature: 25°C to 30°C

Atmosphere: Typical aerobic

18S ribosomal RNA gene, partial sequence; internal   transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA

D1D2 region of the 28S ribosomal RNA gene.

ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGGGGAATGTAGCACTCTTCGGAGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC

28S ribosomal RNA gene and intergenic spacer (IGS), partial sequence

ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAACATGTTGGGTCTCGGGGGGCTTCCTCTAGAGACTTGGATGTAAGGGGCTTTACGCATTCGCATAACTTATGAAGTGTGGATGACTCTAGGAGGGGTCTGAGAATGTGCTGTGCCAGCCAGGCTGATAATAGTTTAATTGTTAGCTTGGACTTGTACACAGTCTCATCAGTCTTCAGCTTGGGTCATTGATATTCTGTAAGTCAAAACTTATCCATTCACTGTAGTAGGTTGCGGAGGACTTGAAAGTTCTCTCTGCAATTGAGACACTTACCAGGCCATCGCAAGTTGGCAGTGTACGCTACTGATTTTATGGTCGTGGGGGACTTGGGAGCTGGTGCTTGTGTCGCATGAGGTAGAGTTGATATGGACGGATGGAAGAGAAGCATATGTGTATGTCGCGGGGGACTTGGGGTAAGACGCCTTGCGGCAAGTAGAGTCAACCAGTAGTGACTTCTTTTAGTCGTGGGGGACTTTGGTAATGGTAGTGAGCCGGAATAAGGGATGGATAAATGTAGTATGGATGGTGACAGGGGGAGCAGCAGAGCGTACGCAGTGGGTATGTATATAGTTGAAAAGGGATGGGCAGTAGAATTTTGAGAAATTAATGACCTAAGGGTGATTCTAATAGGCTTGTCAAGAAAAAAGTTAAGTTGAATCAAGCTGGGTCAAGCAAAGTCTAGAAAAGATTTATTGTCACCAATAATTGCCCAAGTCGCGGGGGGCTTGTCACTTCTCTTGCTCATTGTGGGTCCAGTCT

glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds

TACTTGCATAAATACAATGGTCGTCAAGGTTGGAATCAACGGTTTCGGTACGTAATCGATCCCTCTCTTCCCATTGCTTCATATACCACCGATGTGACTTCCGAATTTGTGCGCTGTTCTTGAGCTACGGTAGCTAACAACTATGTGATAGGTCGTATTGGTCGAATTGTTCTCAGGTATGCTTTTAAATCAACGGGGTAGGACGTCCATGTATCTAACATTCAAGTTAGGAACGCCATCGAGCATGGAGATCTCGAGGTTGTTGCTGTCAACGAGTGAGTGTCCCAAATGTTGACCCATTGGATGAATTACTAAATTGTCCATAGCCCTTTCATTGACTTGGACTACATGGTACGGTACTCCTGTCTGCCCAATAACCTCGTTTAGTACTAACGCTATTGCACAGGTCTACATGTTCAAGTATGACTCCGTAAGTCTTCGATACTTCTAGCGCTCTATATGGCTTATTAACCTGATTCCCGACAGACTCATGGTCGTTTTAAGGGCTCCGTCGAGGTTAAGGACGGTAAGCTTTACATCAACAACAAGGCCATTGCCGTCTTCGGTGAGAAGGACCCTGCCAACATCAAGTGGGGTGAGGCTGGTGCCGAATACGTCGTTGAATCTACCGGTGTTTTCACGACTACCGAAAAGGCCAGTGTTCAT

ATCC

Patient with Hodgkins disease, New York

Nucleotide (GenBank)  :                                KU729082                            ITS including 5.8S rRNA gene

Nucleotide (GenBank)  :                                AF254790                             Filobasidiella neoformans var. neoformans vacuolar (H+)-ATPase

Nucleotide (GenBank)  :                                AF098972                             phosphatidylinositol 3-kinase TOR1 (TOR1), complete coding sequence

Nucleotide (GenBank)  :                                AF097888                             macrolide-binding protein FKBP12 (FRR1) gene, complete coding sequence

Nucleotide (GenBank)  :                                AF097889                             macrolide-binding protein FKBP12 (FRR1) mRNA, complete coding sequence

Nucleotide (GenBank)  :                                AACO02000000                            Cryptococcus neoformans var. grubii H99, whole genome shotgun sequencing project.

Williamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans.  J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929

Feldmesser M, et al. Cryptococcus neoformans is a facultative intracellular pathogen in murine pulmonary infection.  Infect. Immun. 68: 4225-4237, 2000. PubMed: 10858240

del Poeta M, et al. Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein.  Infect. Immun. 67: 1812-1820, 1999. PubMed: 10085022

Franzot SP, et al. Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates.  J. Clin. Microbiol. 37: 838-840, 1999. PubMed: 9986871

Cruz MC, et al. Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans.  Mol. Cell. Biol. 19: 4101-4112, 1999. PubMed: 10330150

Heitman J, et al. The Cryptococcus neoformans genome sequencing project.  Mycopathologia 148: 1-7, 1999.

Peter R Williamson, personal communication

Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot Cell 8(3): 353-361, 2009. PubMed: 19151324

Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262

Ding C, et al. The copper regulon of the human fungal pathogen Cryptococcus neoformans H99. Mol. Microbiol. 81: 1560-1576, 2011. PubMed: 21819456

Meyer W. et al.  Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334

Urai M, et al. Evasion of innate immune responses by the highly virulent Cryptococcus gattii by altering capsule glucuronoxylomannan structure. Front Cell Infect Microbiol 5: 101. doi: 10.3389/fcimb.2015.00101. PubMed: 26779451

Sorrell TC, et al. Cryptococcal transmigration across a model brain blood-barrier: evidence of the Trojan horse mechanism and differences between Cryptococcus neoformans var. grubii strain H99 and Cryptococcus gattii strain R265. Microbes Infect 18: 57-67, 2016. PubMed: 26369713

Movahed E, et al. Genome-wide transcription study of Cryptococcus neoformans H99 clinical strain versus environmental strains. PLoS One 10: e0137457, 2015. PubMed: 26360021

Chang YC, et al. Differences between Cryptococcus neoformans and Cryptococcus gattii in the molecular mechanisms governing utilization of D-amino acids as the sole nitrogen source. PLoS One 10: e0131865, 2015. PubMed: 26132227

Janbon G, et al. Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation. PLoS Genet 10: e1004261, 2014. PubMed: 24743168

Hagen F, et al. Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex. Fungal Genet. Biol. 78: 16-48, 2015. PubMed: 25721988

Peter R Williamson, personal communication