| 产品名称： | pWS10 |
|---|---|
| 商品货号： | TS185833 |
| Designations： | pWS10 |
| GenBank Number： | M24114 |
| Species： | Wound tumor virus |
| Depositors： | DL Nuss |
| Vector： | Construct size (kb): 4.039999961853027 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.169999957084656 Gene product: segment S10, Pns11 polypeptide |
| Insert Size (kb)： | 1.170 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | HindIII cuts the insert twice, 7 bp apart. The insert contains internal EcoRI, AvaI, SalI, AccI, HincII, PstI and HindIII sites. Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.2; EcoRI--3.1, 0.83, 0.41; HindIII--3.9, 0.38; PstI--3.4, 0.92; XbaI--4.3. Linearize the plasmid with SphI for in vitro transcription. This is a full-length clone of virus segment S10. The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1. |
| Classification： | Phytoreovirus |
| References： | Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991 Donald L Nuss, personal communication |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | pWS10 |
|---|---|
| 商品货号： | TS185833 |
| Designations： | pWS10 |
| GenBank Number： | M24114 |
| Species： | Wound tumor virus |
| Depositors： | DL Nuss |
| Vector： | Construct size (kb): 4.039999961853027 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.169999957084656 Gene product: segment S10, Pns11 polypeptide |
| Insert Size (kb)： | 1.170 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | HindIII cuts the insert twice, 7 bp apart. The insert contains internal EcoRI, AvaI, SalI, AccI, HincII, PstI and HindIII sites. Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.2; EcoRI--3.1, 0.83, 0.41; HindIII--3.9, 0.38; PstI--3.4, 0.92; XbaI--4.3. Linearize the plasmid with SphI for in vitro transcription. This is a full-length clone of virus segment S10. The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1. |
| Classification： | Phytoreovirus |
| References： | Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991 Donald L Nuss, personal communication |
