| 产品名称： | pUC1318 |
|---|---|
| 商品货号： | TS186119 |
| Designations： | pUC1318 |
| Depositors： | R Kay |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7300000190734860 Vector: pUC1318 (plasmid) Promoters: Promoter lac Construction: pUC13, pUC18 Marker(s):ampR Construct size (kb): 2.730000019073486 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.50; HindIII--2.55; KpnI--2.55. A pUC type plasmid vector with polylinker designed for addition of new restriction sites to the ends of DNA fragments. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470 Kay R, McPherson J. Hybrid pUC vectors for addition of new restriction enzyme sites to the ends of DNA fragments. Nucleic Acids Res. 15: 2778, 1987. PubMed: 3031611 |
| Shipped： | freeze-dried |
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| 产品名称： | pUC1318 |
|---|---|
| 商品货号： | TS186119 |
| Designations： | pUC1318 |
| Depositors： | R Kay |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7300000190734860 Vector: pUC1318 (plasmid) Promoters: Promoter lac Construction: pUC13, pUC18 Marker(s):ampR Construct size (kb): 2.730000019073486 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.50; HindIII--2.55; KpnI--2.55. A pUC type plasmid vector with polylinker designed for addition of new restriction sites to the ends of DNA fragments. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470 Kay R, McPherson J. Hybrid pUC vectors for addition of new restriction enzyme sites to the ends of DNA fragments. Nucleic Acids Res. 15: 2778, 1987. PubMed: 3031611 |
| Shipped： | freeze-dried |
