| 产品名称： | pLO1297 plasmid in E. coli |
|---|---|
| 商品货号： | TS186262 |
| Designations： | pLO1297 plasmid in E. coli |
| Species： | Zymomonas mobilis (Lindner) Kluyver and van Niel |
| Depositors： | University of Florida, Gainesville, LO Ingram, University of Florida, Gainesville |
| Applications： | produces protein alcohol dehydrogenase II produces protein pyruvate decarboxylase |
| Vector： | Construct size (kb): 8.5 Vector: pUC18 Vector type: plasmid Vector size (kb): 2.686 Markers: ampR Promoter: lac |
| Insert： | Insert size (kb): unknown Genes: alcohol dehydrogenase II (adhB); pyruvate decarboxylase (pdc) DNA: genomic DNA Source: Zymomonas mobilis CP4 Genbank accession: M15394 (adh gene) |
| Media： | 1227 plus 2% glucose
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze-dried E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--3.2, 3.0, 2.5; EcoRI--8.5; SalI--8.5. The GenBank accession number for the adh insert is M15394. Strains containing pLOI297 (TS186262) appear to produce higher levels of ethanol than strains containing pLOI1308-11 (ATCC 68238). ATCC strains 11303 and 15224 transformed with pLOI297 appear to be the best constructs for ethanol production. The 3.2 kb BamHI/EcoRI fragment is the same as that in pLOI295 (ATCC 68240) and contains the two genes. Constructed by inserting a 2.6 kb SalI fragment from pCOS2EMBL containing tetR into the SalI site of pLOI295 (ATCC 68240). The pdc reading frame begins 163 bp downstream of the lac promoter and ends with two stop codons 85 bp upstream of the adhB reading frame. The adhB gene has a single stop codon followed by a palindromic sequence which serves as a terminator. Expression appears to be regulated by the lac promoter. |
| References： | Ingram LO, et al. Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. US Patent 5,000,000 dated Mar 19 1991 Conway T, et al. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 169: 2591-2597, 1987. PubMed: 3584063 Conway T, et al. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169: 949-954, 1987. PubMed: 3029037 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pLO1297 plasmid in E. coli |
|---|---|
| 商品货号： | TS186262 |
| Designations： | pLO1297 plasmid in E. coli |
| Species： | Zymomonas mobilis (Lindner) Kluyver and van Niel |
| Depositors： | University of Florida, Gainesville, LO Ingram, University of Florida, Gainesville |
| Applications： | produces protein alcohol dehydrogenase II produces protein pyruvate decarboxylase |
| Vector： | Construct size (kb): 8.5 Vector: pUC18 Vector type: plasmid Vector size (kb): 2.686 Markers: ampR Promoter: lac |
| Insert： | Insert size (kb): unknown Genes: alcohol dehydrogenase II (adhB); pyruvate decarboxylase (pdc) DNA: genomic DNA Source: Zymomonas mobilis CP4 Genbank accession: M15394 (adh gene) |
| Media： | 1227 plus 2% glucose
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze-dried E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--3.2, 3.0, 2.5; EcoRI--8.5; SalI--8.5. The GenBank accession number for the adh insert is M15394. Strains containing pLOI297 (TS186262) appear to produce higher levels of ethanol than strains containing pLOI1308-11 (ATCC 68238). ATCC strains 11303 and 15224 transformed with pLOI297 appear to be the best constructs for ethanol production. The 3.2 kb BamHI/EcoRI fragment is the same as that in pLOI295 (ATCC 68240) and contains the two genes. Constructed by inserting a 2.6 kb SalI fragment from pCOS2EMBL containing tetR into the SalI site of pLOI295 (ATCC 68240). The pdc reading frame begins 163 bp downstream of the lac promoter and ends with two stop codons 85 bp upstream of the adhB reading frame. The adhB gene has a single stop codon followed by a palindromic sequence which serves as a terminator. Expression appears to be regulated by the lac promoter. |
| References： | Ingram LO, et al. Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. US Patent 5,000,000 dated Mar 19 1991 Conway T, et al. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 169: 2591-2597, 1987. PubMed: 3584063 Conway T, et al. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169: 949-954, 1987. PubMed: 3029037 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
