你好，请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
你好,请登录   免费注册 | 收藏
联系电话: call_new 0574-87917803
热门搜索: 金黄色葡萄球菌 铜绿假单胞菌 红菇 香菇
Naegleria lovaniensis Steven et al.
Naegleria lovaniensis Steven et al.
规格:
货期:
编号:TS186401
品牌:Testobio
产品名称： Naegleria lovaniensis Steven et al.
商品货号： TS186401
Deposited As： Naegleria fowleri Carter
Strain Designations： K-1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Domestic water supply, Kadina, Australia, 1972
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Comments：
Received axenic, distributed monoxenic with Escherichia coli ATCC 11775
Medium： ATCC® Medium 997: Fresh water ameba medium
ATCC® Medium 711: PYB
Growth Conditions：
Temperature: 35°C
Culture System: Xenic
Cryopreservation： Reagents
Cryoprotective Solution
DMSO 1.5 mL
Pages Balanced Salt Solution (or similar) 8.5 mL

Harvest and Preservation

  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL ATCC medium 1323 (Pages Balanced Salt Solution) and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.)xa0xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 997.xa0 Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 35°C.
  10. Follow the protocol for maintenance of culture.
Name of Depositor： JA Jamieson
Year of Origin： 1972
References：

Anderson K, Jamieson A. Bacterial suspensions for the growth of Naegleria species. Pathology 6: 79-84, 1974. PubMed: 4207323

De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.

首页 > 产品中心 > 微生物培养 > 菌株 > null > Naegleria lovaniensis Steven et al.

产品中心 Product Center

联系我们CONTACT US

  • 0574-87917803
    24小时服务热线
  • testobio@163.com
    销售邮箱
  • 浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
    地址

联系方式

  • 章丽敏
    17757489010
  • 朱静
    18067106081
  • 李晴
    18067408036
  • 李晶晶
    13375187189
  • 周雪
    13372109301

Naegleria lovaniensis Steven et al.

  • 货号: TS186401
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Naegleria lovaniensis Steven et al.
商品货号： TS186401
Deposited As： Naegleria fowleri Carter
Strain Designations： K-1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Domestic water supply, Kadina, Australia, 1972
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Comments：
Received axenic, distributed monoxenic with Escherichia coli ATCC 11775
Medium： ATCC® Medium 997: Fresh water ameba medium
ATCC® Medium 711: PYB
Growth Conditions：
Temperature: 35°C
Culture System: Xenic
Cryopreservation： Reagents
Cryoprotective Solution
DMSO 1.5 mL
Pages Balanced Salt Solution (or similar) 8.5 mL

Harvest and Preservation

  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL ATCC medium 1323 (Pages Balanced Salt Solution) and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.)xa0xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 997.xa0 Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 35°C.
  10. Follow the protocol for maintenance of culture.
Name of Depositor： JA Jamieson
Year of Origin： 1972
References：

Anderson K, Jamieson A. Bacterial suspensions for the growth of Naegleria species. Pathology 6: 79-84, 1974. PubMed: 4207323

De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.

testopic
小红书
扫码关注小红书账号
关注我们
联系电话：0574-87917803
章丽敏：17757489010
朱静：18067106081
李晴：18067408036
李晶晶：13375187189
周雪：13372109301
邮箱：testobio@163.com
地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
在线留言
合作单位: