| 产品名称： | DG-75 D.G.-75 |
|---|---|
| 商品货号： | TS186486 |
| Organism： | Homo sapiens, human |
| Tissue： | derived from metastatic site: pleural effusion |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 10 years |
| Gender： | male |
| Ethnicity： | White |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | t(8:14)(q24;q32)
Ref  Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Derivation： | The DG-75 cell line was established in 1975 from a pleural effusion of a patient with a primary abdominal lymphoma, which clinically and histologically resembled Burkitts lymphoma. |
| Antigen Expression： | CD10; Homo sapiens, expressed Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273CD19; Homo sapiens, expressed (weakly expressed) Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273(weakly expressed)CD20; Homo sapiens Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273CD23; Homo sapiens Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273CD38; Homo sapiens, expressed Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273CD39; Homo sapiens Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Receptor Expression： | Fc, not expressed Ref Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769complement (C3), not expressed Ref Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769 |
| Genes Expressed： | surface immunoglobulin (IgM, k)xa0Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Cellular Products： | surface immunoglobulin (IgM, k)
Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Comments： | The cells are reported to be negative for Epstein-Barr virus and E-rosette negative.xa0RefBen-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769
A culture submitted to the ATCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. After a six-week period following treatment, the cells were assayed for mycoplasma, by Hoechst stain, PCR and by the standard culture test. All tests were negative.
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 3 x 105 and 3 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
|
| Population Doubling Time： | 20 to 24 hrs |
| Name of Depositor： | H Ben-Bassat |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1975 |
| References： | Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769 Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
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| 产品名称： | DG-75 D.G.-75 |
|---|---|
| 商品货号： | TS186486 |
| Organism： | Homo sapiens, human |
| Tissue： | derived from metastatic site: pleural effusion |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 10 years |
| Gender： | male |
| Ethnicity： | White |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | t(8:14)(q24;q32)
RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Derivation： | The DG-75 cell line was established in 1975 from a pleural effusion of a patient with a primary abdominal lymphoma, which clinically and histologically resembled Burkitts lymphoma. |
| Antigen Expression： | CD10; Homo sapiens, expressed Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273CD19; Homo sapiens, expressed (weakly expressed) Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273(weakly expressed)CD20; Homo sapiens Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273CD23; Homo sapiens Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273CD38; Homo sapiens, expressed Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273CD39; Homo sapiens Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Receptor Expression： | Fc, not expressed Ref Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769complement (C3), not expressed Ref Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769 |
| Genes Expressed： | surface immunoglobulin (IgM, k)xa0Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Cellular Products： | surface immunoglobulin (IgM, k)
Ref Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Comments： | The cells are reported to be negative for Epstein-Barr virus and E-rosette negative.xa0RefBen-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769
A culture submitted to the ATCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. After a six-week period following treatment, the cells were assayed for mycoplasma, by Hoechst stain, PCR and by the standard culture test. All tests were negative.
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 3 x 105 and 3 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
|
| Population Doubling Time： | 20 to 24 hrs |
| Name of Depositor： | H Ben-Bassat |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1975 |
| References： | Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769 Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
