| 产品名称： | pGEM4Z/CREB2 |
|---|---|
| 商品货号： | TS186509 |
| Designations： | pGEM4Z/CREB2 |
| GenBank Number： | M86842 |
| Species： | Homo sapiens, human |
| Depositors： | JM Leiden |
| Applications： | in another host, produces protein cAMP responsive element binding protein 2 |
| Vector： | Construct size (kb): 5.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-4Z Intact vector size: 3.000 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CREB2 Gene name: cAMP responsive element binding protein 2 Contains complete coding sequence?: U Chromosome: 2; Localization: 2 q32 Tissue: acute T cell leukemia, Jurkat cell line Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.3 Cross references: DNA Seq. Acc.: M86842 Insert lengths(kb): 1.299999952316284 Tissue: acute T cell leukemia, Jurkat cell line Gene product: cAMP responsive element binding protein 2 CREB2 Target Gene: cAMP responsive element binding protein 2 |
| Insert Size (kb)： | 1.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 1.40; KpnI--4.2, 1.05; PstI--5.3; BamHI--5.3; EcoRV--3.8, 0.72, 0.45. The insert contains the following restriction sites (nt from the 5 EcoRI site): XmnI--383; EcoRV--667; KpnI--991. Detects a transcript of 1.5 kb in human cells. Has also been used to detect mouse transcripts. |
| References： | Karpinski BA, et al. Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element. Proc. Natl. Acad. Sci. USA 89: 4820-4824, 1992. PubMed: 1534408 Jeffrey M Leiden, personal communication |
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| 产品名称： | pGEM4Z/CREB2 |
|---|---|
| 商品货号： | TS186509 |
| Designations： | pGEM4Z/CREB2 |
| GenBank Number： | M86842 |
| Species： | Homo sapiens, human |
| Depositors： | JM Leiden |
| Applications： | in another host, produces protein cAMP responsive element binding protein 2 |
| Vector： | Construct size (kb): 5.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-4Z Intact vector size: 3.000 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CREB2 Gene name: cAMP responsive element binding protein 2 Contains complete coding sequence?: U Chromosome: 2; Localization: 2 q32 Tissue: acute T cell leukemia, Jurkat cell line Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.3 Cross references: DNA Seq. Acc.: M86842 Insert lengths(kb): 1.299999952316284 Tissue: acute T cell leukemia, Jurkat cell line Gene product: cAMP responsive element binding protein 2 CREB2 Target Gene: cAMP responsive element binding protein 2 |
| Insert Size (kb)： | 1.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 1.40; KpnI--4.2, 1.05; PstI--5.3; BamHI--5.3; EcoRV--3.8, 0.72, 0.45. The insert contains the following restriction sites (nt from the 5 EcoRI site): XmnI--383; EcoRV--667; KpnI--991. Detects a transcript of 1.5 kb in human cells. Has also been used to detect mouse transcripts. |
| References： | Karpinski BA, et al. Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element. Proc. Natl. Acad. Sci. USA 89: 4820-4824, 1992. PubMed: 1534408 Jeffrey M Leiden, personal communication |
