k2.8
k2.8
规格:
货期:
编号:TS186948
品牌:Testobio
| 产品名称： | k2.8 |
|---|---|
| 商品货号： | TS186948 |
| Designations： | k2.8 |
| GenBank Number： | L13974 |
| Species： | Homo sapiens, human |
| Depositors： | P Ney |
| Vector： | Construct size (kb): 4.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: NFE2 Gene name: nuclear factor (erythroid-derived 2), 45kD Contains complete coding sequence?: U Chromosome: 12; Localization: 12 q13 Tissue: K562 cells Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 1.496 Cross references: DNA Seq. Acc.: L13974 Nucleotides 1-1496 of the insert correspond to nucleotides 1-1496 of L13974. Insert lengths(kb): 1.496000051498413 Tissue: K562 cells Gene product: nuclear factor (erythroid-derived 2), 45kD(nuclear factor 45) NFE2 |
| Insert Size (kb)： | 1.496 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4; EcoRI--2.7, 1.8; KpnI--3.3, 1.2; PstI--4.4; SalI--3.0, 1.3. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.10; BglI--0.21, 0.60, 0.65; KpnI--1.02; PstI--0.37; PvuII--0.30, 0.85, 1.13, 1.23; SacI--0.51; SmaI--0.06, 1.35; XbaI--0.20, 1.47. |
| References： | Ney PA, et al. Purification of the human NF-E2 complex: cDNA cloning of the hematopoietic cell-specific subunit and evidence for an associated partner. Mol. Cell. Biol. 13: 5604-5612, 1993. PubMed: 8355703 |
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| 产品名称： | k2.8 |
|---|---|
| 商品货号： | TS186948 |
| Designations： | k2.8 |
| GenBank Number： | L13974 |
| Species： | Homo sapiens, human |
| Depositors： | P Ney |
| Vector： | Construct size (kb): 4.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: NFE2 Gene name: nuclear factor (erythroid-derived 2), 45kD Contains complete coding sequence?: U Chromosome: 12; Localization: 12 q13 Tissue: K562 cells Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 1.496 Cross references: DNA Seq. Acc.: L13974 Nucleotides 1-1496 of the insert correspond to nucleotides 1-1496 of L13974. Insert lengths(kb): 1.496000051498413 Tissue: K562 cells Gene product: nuclear factor (erythroid-derived 2), 45kD(nuclear factor 45) NFE2 |
| Insert Size (kb)： | 1.496 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4; EcoRI--2.7, 1.8; KpnI--3.3, 1.2; PstI--4.4; SalI--3.0, 1.3. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.10; BglI--0.21, 0.60, 0.65; KpnI--1.02; PstI--0.37; PvuII--0.30, 0.85, 1.13, 1.23; SacI--0.51; SmaI--0.06, 1.35; XbaI--0.20, 1.47. |
| References： | Ney PA, et al. Purification of the human NF-E2 complex: cDNA cloning of the hematopoietic cell-specific subunit and evidence for an associated partner. Mol. Cell. Biol. 13: 5604-5612, 1993. PubMed: 8355703 |
