| 产品名称： | phTGF1-10-925 |
|---|---|
| 商品货号： | TS187068 |
| Designations： | phTGF1-10-925 |
| GenBank Number： | X70340 |
| Species： | Homo sapiens, human |
| Depositors： | GI Bell |
| Vector： | Construct size (kb): 4.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.925000011920929 Tissue: kidney Gene product: transforming growth factor, alpha(transforming growth factor alpha) TGFA Alleles: A1, A2, B1, B2 |
| Insert Size (kb)： | 0.925 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following restriction sites, in bp from the 5 end of the insert: PstI (167), PstI (234), RsaI (285), XmnI (590). Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 0.9; BglI--1.5, 1.1 (doublet), 0.3; SphI--2.9, 1.1; BamHI--4.3; PvuI--4.3. The insert includes 49 bp of 5 untranslated, 480 bp encoding transforming growth factor alpha precursor, and 391 bp 3 untranslated. The RsaI polymorphism is in linkage disequilibrium with a BamHI polymorphism detected by phTGF1-10-3350, (r=0.55, p=0.03). Enzyme(s) not detecting polymorphism: BclII, BglII, EcoRI, EcoRV, HaeIII, HindIII, HinfI, KpnI, MspI, PstI, PvuII, SacI, XbaI, XhoI. |
| References： | Tricoli JV, et al. The gene for human transforming growth factor alpha is on the short arm of chromosome 2. Cytogenet. Cell Genet. 42: 94-98, 1986. PubMed: 3459638 Murray JC, et al. RFLPs for transforming growth factor alpha (TGFA) gene at 2p13. Nucleic Acids Res. 14: 7136, 1986. PubMed: 2876411 Murray JC, et al. Linkage of an autosomal dominant clefting syndrome (Van der Woude) to loci on chromosome 1q. Am. J. Hum. Genet. 46: 486-491, 1990. PubMed: 2309700 Graeme I Bell, personal communication |
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| 产品名称： | phTGF1-10-925 |
|---|---|
| 商品货号： | TS187068 |
| Designations： | phTGF1-10-925 |
| GenBank Number： | X70340 |
| Species： | Homo sapiens, human |
| Depositors： | GI Bell |
| Vector： | Construct size (kb): 4.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.925000011920929 Tissue: kidney Gene product: transforming growth factor, alpha(transforming growth factor alpha) TGFA Alleles: A1, A2, B1, B2 |
| Insert Size (kb)： | 0.925 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following restriction sites, in bp from the 5 end of the insert: PstI (167), PstI (234), RsaI (285), XmnI (590). Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 0.9; BglI--1.5, 1.1 (doublet), 0.3; SphI--2.9, 1.1; BamHI--4.3; PvuI--4.3. The insert includes 49 bp of 5 untranslated, 480 bp encoding transforming growth factor alpha precursor, and 391 bp 3 untranslated. The RsaI polymorphism is in linkage disequilibrium with a BamHI polymorphism detected by phTGF1-10-3350, (r=0.55, p=0.03). Enzyme(s) not detecting polymorphism: BclII, BglII, EcoRI, EcoRV, HaeIII, HindIII, HinfI, KpnI, MspI, PstI, PvuII, SacI, XbaI, XhoI. |
| References： | Tricoli JV, et al. The gene for human transforming growth factor alpha is on the short arm of chromosome 2. Cytogenet. Cell Genet. 42: 94-98, 1986. PubMed: 3459638 Murray JC, et al. RFLPs for transforming growth factor alpha (TGFA) gene at 2p13. Nucleic Acids Res. 14: 7136, 1986. PubMed: 2876411 Murray JC, et al. Linkage of an autosomal dominant clefting syndrome (Van der Woude) to loci on chromosome 1q. Am. J. Hum. Genet. 46: 486-491, 1990. PubMed: 2309700 Graeme I Bell, personal communication |
