pFa6a-KANMX6-pADH1-GST
pFa6a-KANMX6-pADH1-GST
规格:
货期:
编号:TS187292
品牌:Testobio
| 产品名称： | pFa6a-KANMX6-pADH1-GST |
|---|---|
| 商品货号： | TS187292 |
| Designations： | pFa6a-KANMX6-pADH1-GST |
| Depositors： | DJ DeMarini |
| Biosafety Level： | 1 |
| Vector Information： | Vector: pFa6a-KANMX6-pADH1-GST (plasmid) Construction: pCRTOPO2.1 Marker(s):ampR Construct size (kb): 5.557000160217285 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--5.6; EcoRI--5.6; BglII/EcoRI--4.4, 1.3. The marker, promoter and GST cassette is flanked by the F4 primer site (5) and the R4 primer site (3). The size of the F4/R4 PCR product is 2692 nt. F4 primer sequence: 5-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3. R6 primer sequence: 5-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3. R4 primer sequence: 5-(gene-specific sequence) ACGCGGAACCAGATCCGATT-3. The gene-specific sequences of these primers are chosen to correspond to the reverse complement of the N-terminal codons of the target gene. Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899 |
| Related Products： | component of:ATCC 87819 |
| Shipped： | freeze-dried |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
pFa6a-KANMX6-pADH1-GST
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | pFa6a-KANMX6-pADH1-GST |
|---|---|
| 商品货号： | TS187292 |
| Designations： | pFa6a-KANMX6-pADH1-GST |
| Depositors： | DJ DeMarini |
| Biosafety Level： | 1 |
| Vector Information： | Vector: pFa6a-KANMX6-pADH1-GST (plasmid) Construction: pCRTOPO2.1 Marker(s):ampR Construct size (kb): 5.557000160217285 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--5.6; EcoRI--5.6; BglII/EcoRI--4.4, 1.3. The marker, promoter and GST cassette is flanked by the F4 primer site (5) and the R4 primer site (3). The size of the F4/R4 PCR product is 2692 nt. F4 primer sequence: 5-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3. R6 primer sequence: 5-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3. R4 primer sequence: 5-(gene-specific sequence) ACGCGGAACCAGATCCGATT-3. The gene-specific sequences of these primers are chosen to correspond to the reverse complement of the N-terminal codons of the target gene. Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899 |
| Related Products： | component of:ATCC 87819 |
| Shipped： | freeze-dried |
