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pCK92 [hMCT1]
pCK92 [hMCT1]
规格:
货期:
编号:TS187421
品牌:Testobio
产品名称: pCK92 hMCT1
商品货号: TS187421
Designations: pCK92 hMCT1
GenBank Number:

L31801

Species: Homo sapiens, human
Depositors: JL Goldstein
Vector:
Construct size (kb): 5.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: SalI
Vector end: PstI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SLC16A1
Gene name: solute carrier family 16 (monocarboxylate transporters),
member 1
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 p13.2-p12
Tissue: heart
Type of DNA: cDNA
Insert 5 end: SalI
Insert 3 end: PstI
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: L31801
Insert lengths(kb): 2.5
Tissue: heart
Gene product: mevalonate transporter(monocarboxylate transporter 1) SLC16A1
Insert Size (kb): 2.5
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRV--4.1, 1.4; PstI--5.5; PstI/SalI--2.9, 2.6; XhoI--5.5; BamHI--5.5.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--0.2, 1.6.
Contains the complete coding sequence plus the 3 untranslated region of approximately 1 kb.
References:

Garcia CK, et al. cDNA cloning of the human monocarboxylate transporter 1 and chromosomal localization of the SLC16A1 locus to 1p13.2-p12. Genomics 23: 500-503, 1994. PubMed: 7835905

Joseph L Goldstein, personal communication

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pCK92 [hMCT1]

  • 货号: TS187421
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: pCK92 hMCT1
商品货号: TS187421
Designations: pCK92 hMCT1
GenBank Number:

L31801

Species: Homo sapiens, human
Depositors: JL Goldstein
Vector:
Construct size (kb): 5.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: SalI
Vector end: PstI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SLC16A1
Gene name: solute carrier family 16 (monocarboxylate transporters),
member 1
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 p13.2-p12
Tissue: heart
Type of DNA: cDNA
Insert 5 end: SalI
Insert 3 end: PstI
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: L31801
Insert lengths(kb): 2.5
Tissue: heart
Gene product: mevalonate transporter(monocarboxylate transporter 1) SLC16A1
Insert Size (kb): 2.5
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRV--4.1, 1.4; PstI--5.5; PstI/SalI--2.9, 2.6; XhoI--5.5; BamHI--5.5.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--0.2, 1.6.
Contains the complete coding sequence plus the 3 untranslated region of approximately 1 kb.
References:

Garcia CK, et al. cDNA cloning of the human monocarboxylate transporter 1 and chromosomal localization of the SLC16A1 locus to 1p13.2-p12. Genomics 23: 500-503, 1994. PubMed: 7835905

Joseph L Goldstein, personal communication

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