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pLAFR1 cosmid in E. coli
pLAFR1 cosmid in E. coli
规格:
货期:
编号:TS187562
品牌:Testobio
产品名称: pLAFR1 cosmid in E. coli
商品货号: TS187562
Designations: pLAFR1 cosmid in E. coli
Depositors: FM Ausubel
Biosafety Level: 1
Vector Information:
Size (kb): 21.60
Vector: pLAFR1 (cosmid)
Construction: pRK270, pHC79
Marker(s):tetR
Construct size (kb): 21.60
Features: marker(s): tetR
replicon: RK2, pMB1
Applications:
shuttle vector
vector for constructing genomic libraries
Comments:
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + tet plates at 30C and picking small colonies for liquid culture.
A broad host range cosmid vector containing a relaxation complex site.
Media: ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions:
Temperature: 30.0°C
References:

Friedman AM, et al. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18: 289-296, 1982. PubMed: 6290332

Shipped: freeze-dried
Shipping Information:

Shipped: xa0Freeze dried E. coli containing the cosmid

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pLAFR1 cosmid in E. coli

  • 货号: TS187562
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询价
  • 品牌 : TESTOBIO
产品名称: pLAFR1 cosmid in E. coli
商品货号: TS187562
Designations: pLAFR1 cosmid in E. coli
Depositors: FM Ausubel
Biosafety Level: 1
Vector Information:
Size (kb): 21.60
Vector: pLAFR1 (cosmid)
Construction: pRK270, pHC79
Marker(s):tetR
Construct size (kb): 21.60
Features: marker(s): tetR
replicon: RK2, pMB1
Applications:
shuttle vector
vector for constructing genomic libraries
Comments:
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + tet plates at 30C and picking small colonies for liquid culture.
A broad host range cosmid vector containing a relaxation complex site.
Media: ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions:
Temperature: 30.0°C
References:

Friedman AM, et al. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18: 289-296, 1982. PubMed: 6290332

Shipped: freeze-dried
Shipping Information:

Shipped: xa0Freeze dried E. coli containing the cosmid

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