Bad KO SV40 MEF

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货期:

编号:TS187634

品牌:Testobio

产品名称：	Bad KO SV40 MEF
商品货号：	TS187634
Organism：	Mus musculus, mouse
Tissue：	embryonic fibroblast
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast-like
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications：	This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, and BAD function.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Cells are immortalized MEFs generated from BAD knockout mice.
Tumorigenic：	no
Comments：	Cells are immortalized MEFs (genotype:xa0bad^-/-), which werexa0generated from BAD knockout mice.xa0 BAD is a pro-apoptotic BCL-2 family member (“BH3-only” subset) and is regulated by phosphorylation in response to growth/survival factors This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, and BAD function.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated IMDM Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 10 % Fetal Bovine Serum and 1x Non-essential amino acids.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 7 X 10³ to 1.5 X 10⁴ viable cells/cm² is recommended. Incubate cultures at 37°C. At subculture, cell concentration is between 8 X 10⁴ to 1.5 X 10⁵ cells/cm². Subcultivation ratio: A subcultivation ratio of 1:6 to 1:12 is recommended. Medium renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: 90% Fetal Bovine Serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor：	S Korsmeyer
References：	Danial N, et al. BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 424 (6951) : 952-6, 2003 PubMed: 12931191 Cheng EH, et al. BCL-2, BCL-XL Sequester BH3 Domain-Only Molecules Preventing BAX- and BAK-Mediated Mitochondrial Apoptosis. Molecular Cell 8(3) : 705–711, 2001 PubMed: 11583631

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Bad KO SV40 MEF

货号: TS187634
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产品名称：	Bad KO SV40 MEF
商品货号：	TS187634
Organism：	Mus musculus, mouse
Tissue：	embryonic fibroblast
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast-like
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications：	This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, and BAD function.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Cells are immortalized MEFs generated from BAD knockout mice.
Tumorigenic：	no
Comments：	Cells are immortalized MEFs (genotype:xa0bad^-/-), which werexa0generated from BAD knockout mice.xa0 BAD is a pro-apoptotic BCL-2 family member (“BH3-only” subset) and is regulated by phosphorylation in response to growth/survival factors This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, and BAD function.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated IMDM Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 10 % Fetal Bovine Serum and 1x Non-essential amino acids.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 7 X 10³ to 1.5 X 10⁴ viable cells/cm² is recommended. Incubate cultures at 37°C. At subculture, cell concentration is between 8 X 10⁴ to 1.5 X 10⁵ cells/cm². Subcultivation ratio: A subcultivation ratio of 1:6 to 1:12 is recommended. Medium renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: 90% Fetal Bovine Serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor：	S Korsmeyer
References：	Danial N, et al. BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 424 (6951) : 952-6, 2003 PubMed: 12931191 Cheng EH, et al. BCL-2, BCL-XL Sequester BH3 Domain-Only Molecules Preventing BAX- and BAK-Mediated Mitochondrial Apoptosis. Molecular Cell 8(3) : 705–711, 2001 PubMed: 11583631